公开(公告)号：US20240103009A1

公开(公告)日：2024-03-28

申请号：US18275855

申请日：2022-02-04

Applicant: AMGEN INC.

Inventor： Karyn E. MCFADDEN ,  Brian M. CHAN ,  Christopher MURAWSKY ,  Aaron George WINTERS ,  Daniel SANTOS

IPC: G01N33/68 ,  C07K16/00 ,  C12N5/0781

CPC classification number: G01N33/6854 ,  C07K16/00 ,  C12N5/0635 ,  C07K2317/14 ,  C07K2317/92

Abstract: Provided herein are methods of monitoring for the production of select antibodies in a non-human animal, comprising (a) immunizing a non-human animal with an immunogen; (b) obtaining a blood sample comprising antibody secreting cells (ASCs) from said non-human animal; and (c) individually assaying ASCs present in the blood sample, or a fraction thereof, for the production of select antibodies. Methods of guiding antibody production in a non-human animal for the production of select antibodies are also provided. In exemplary embodiments, the method comprises performing a cycle of (a) to (c), as above, and repeating the cycle when the percentage of ASCs producing select antibodies is below a threshold. In various aspects, the cycle is repeated until the percentage of ASCs producing select antibodies is at or above a threshold. Single cell assays are further provided herein.