公开(公告)号：US20240103009A1

公开(公告)日：2024-03-28

申请号：US18275855

申请日：2022-02-04

Applicant: AMGEN INC.

Inventor： Karyn E. MCFADDEN ,  Brian M. CHAN ,  Christopher MURAWSKY ,  Aaron George WINTERS ,  Daniel SANTOS

IPC: G01N33/68 ,  C07K16/00 ,  C12N5/0781

CPC classification number: G01N33/6854 ,  C07K16/00 ,  C12N5/0635 ,  C07K2317/14 ,  C07K2317/92

Abstract: Provided herein are methods of monitoring for the production of select antibodies in a non-human animal, comprising (a) immunizing a non-human animal with an immunogen; (b) obtaining a blood sample comprising antibody secreting cells (ASCs) from said non-human animal; and (c) individually assaying ASCs present in the blood sample, or a fraction thereof, for the production of select antibodies. Methods of guiding antibody production in a non-human animal for the production of select antibodies are also provided. In exemplary embodiments, the method comprises performing a cycle of (a) to (c), as above, and repeating the cycle when the percentage of ASCs producing select antibodies is below a threshold. In various aspects, the cycle is repeated until the percentage of ASCs producing select antibodies is at or above a threshold. Single cell assays are further provided herein.

公开(公告)号：US20220226829A1

公开(公告)日：2022-07-21

申请号：US17616616

申请日：2020-06-04

Applicant: Amgen Inc.

Inventor： Craig Michael SCHULZ ,  Justin James PROVCHY ,  Aaron George WINTERS

IPC: B01L5/00

Abstract: Systems for performing cellular analysis and related devices for conditioning environments adjacent chips in such systems. A device for conditioning an environment adjacent a chip in a system for performing cellular analysis, the device includes a cover for being disposed adjacent the chip and comprising a planar body having a top surface, a bottom surface, and an outer edge surface. The cover includes a central opening extending between the top surface and the bottom surface and bounded by an inner edge surface of the cover. The cover also includes a fluid inlet extending into the body from the outer edge surface between the top surface and the bottom surface the fluid inlet arranged to accept a gas to be delivered to the central opening. The cover also includes a plurality of fluid outlets defined in the inner edge surface and in fluid communication with the fluid inlet. The plurality of fluid outlets are arranged to receive the gas from the fluid inlet and exhaust the gas into the central opening.