公开(公告)号：US20160193295A1

公开(公告)日：2016-07-07

申请号：US14909431

申请日：2014-07-30

Applicant: AMGEN INC.

Inventor： Gunasekaran KANNAN ,  Jennifer LAVALLEE ,  Frederick W. JACOBSEN

IPC: A61K38/18 ,  A61K47/48 ,  C07K14/475

CPC classification number: A61K38/18 ,  A61K47/48376 ,  A61K47/6801 ,  C07K14/475 ,  C07K16/00 ,  C07K2317/52 ,  C07K2317/526 ,  C07K2319/30

Abstract: This disclosure provides polypeptides comprising an antibody Fc region having a deletion of one or more cysteine residues in the hinge region and substitution with a sulfhydryl-containing residue of one or more CH3-interface amino acids. Also, provided are Fc-fusion proteins and antibodies containing said polypeptides, nucleic acids and vectors encoding said polypeptides, along with host cells and methods for making said polypeptides.

Abstract translation: 本公开提供了包含在铰链区中具有一个或多个半胱氨酸残基缺失的抗体Fc区并用一个或多个CH 3界面氨基酸的含巯基残基取代的多肽。 此外，提供了Fc融合蛋白和含有所述多肽，核酸和编码所述多肽的载体以及宿主细胞的Fc融合蛋白和制备所述多肽的方法。

公开(公告)号：US20190192628A1

公开(公告)日：2019-06-27

申请号：US16297494

申请日：2019-03-08

Applicant: AMGEN INC.

Inventor： Gunasekaran KANNAN ,  Jennifer LAVALLEE ,  Frederick W. JACOBSEN

IPC: A61K38/18 ,  C07K16/00 ,  A61K47/68 ,  C07K14/475

CPC classification number: A61K38/18 ,  A61K47/48376 ,  A61K47/6801 ,  C07K14/475 ,  C07K16/00 ,  C07K2317/52 ,  C07K2317/526 ,  C07K2319/30

Abstract: This disclosure provides polypeptides comprising an antibody Fc region having a deletion of one or more cysteine residues in the hinge region and substitution with a sulfhydryl-containing residue of one or more CH3-interface amino acids. Also, provided are Fc-fusion proteins and antibodies containing said polypeptides, nucleic acids and vectors encoding said polypeptides, along with host cells and methods for making said polypeptides.