公开(公告)号：US20240118200A1

公开(公告)日：2024-04-11

申请号：US18265057

申请日：2021-12-06

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Runli LIANG ,  Guangzhong MA

IPC: G01N21/43 ,  G01N33/542

CPC classification number: G01N21/43 ,  G01N33/542 ,  G01N2021/437

Abstract: This disclosure describes systems and methods for critical angle reflection (CAR) imaging to quantify molecular binding kinetics on a glass surface in some embodiments. CAR is a label-free method that measures the reflectivity change near a critical angle in response to molecular binding induced refractive index changes on the sensor surface. The sensitivity and dynamic range of CAR is tunable by varying the incident angle of light, which allows for optimizing the measurement for ligands with different sizes in both biomolecular and cell-based studies.