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1.发明申请公开(公告)号：US20220243246A1
公开(公告)日：2022-08-04
申请号：US17590457
申请日：2022-02-01
Inventor： Shaopeng WANG , Fenni ZHANG
Abstract: Provided herein are methods of assessing the presence of microbes in a liquid sample that include assessing an initial integrated scattering intensity of objects (IC0) in the sample and an integrated scattering intensity of the objects at a time t (ICt) from modified images of the liquid sample, and identifying the sample as comprising microbes for (ICt)/(IC0) above a predefined infection threshold TI. Related systems and other aspects are also provided.
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公开(公告)号：US20250035627A1
公开(公告)日：2025-01-30
申请号：US18778278
申请日：2024-07-19
Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , Mayo Foundation for Medical Education and Research
Inventor： Shaopeng WANG , Nanxi YU , Chao CHEN , Xinyu ZHOU , Januario E. CASTRO , Eider F. Moreno Cortes
IPC: G01N33/569 , B01L3/00 , C12N5/00 , G01N33/543 , G01N33/68 , G06V10/70
Abstract: Provided herein are methods of differentiating cell types in a cell population. The methods include removing at least some non-Chimeric Antigen Receptor (CAR)-T cells from a fluidic sample obtained from a subject without centrifuging the fluidic sample to produce a purified fluidic sample. The fluidic sample comprises CAR-T cells and the non-CAR-T cells. The methods also include capturing cells in the purified fluidic sample on a surface that comprises binding moieties that bind at least to the CAR-T cells to produce a captured cell population. In addition, the methods also include distinguishing the CAR-T cells from the non-CAR-T cells in the captured cell population using a trained machine learning model to produce a captured CAR-T cell population data set. Additional methods as well as related devices and systems are also provided.
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公开(公告)号：US20230408409A1
公开(公告)日：2023-12-21
申请号：US18204735
申请日：2023-06-01
Inventor： Shaopeng WANG , Pengfei ZHANG
IPC: G01N21/55 , G01N33/543 , G16B15/00
CPC classification number: G01N21/55 , G01N33/54373 , G16B15/00 , G01N2021/556
Abstract: Provided herein are methods of detecting exosomes, including unlabeled exosomes. In some embodiments, the methods include disposing a fluidic sample that comprises a plurality of exosomes in a chamber that is positioned at least partially within a fluidic device in which an inner surface of the chamber comprises a first set of exosome binding moieties that are capable of binding the exosomes. In some embodiments, the methods also include binding a portion of the plurality of exosomes to a portion of the first set of exosome binding moieties to produce surface-bound exosomes, introducing an incident light toward the inner surface of the chamber prior to, concurrent with, and/or after, producing the surface-bound exosomes, and detecting light scattered by the surface-bound exosomes to produce a set of exosome imaging data. Related fluidic devices, systems, and computer readable media are also provided.
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公开(公告)号：US20240118200A1
公开(公告)日：2024-04-11
申请号：US18265057
申请日：2021-12-06
Inventor： Shaopeng WANG , Runli LIANG , Guangzhong MA
IPC: G01N21/43 , G01N33/542
CPC classification number: G01N21/43 , G01N33/542 , G01N2021/437
Abstract: This disclosure describes systems and methods for critical angle reflection (CAR) imaging to quantify molecular binding kinetics on a glass surface in some embodiments. CAR is a label-free method that measures the reflectivity change near a critical angle in response to molecular binding induced refractive index changes on the sensor surface. The sensitivity and dynamic range of CAR is tunable by varying the incident angle of light, which allows for optimizing the measurement for ligands with different sizes in both biomolecular and cell-based studies.
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公开(公告)号：US20230288331A1
公开(公告)日：2023-09-14
申请号：US18180228
申请日：2023-03-08
Inventor： Shaopeng WANG , Yunlei ZHAO , Guangzhong MA
CPC classification number: G01N21/51 , B82Y15/00 , G01N2201/0461 , G01N2201/0484 , G01N2201/067
Abstract: Provided herein are methods of determining molecular binding kinetics on particles, such as magnetic nanoparticles. In some embodiments, the methods include introducing an incident light from a light source toward a sample container that comprises a particle-bound biomolecule-ligand composition comprising a plurality of particle-bound biomolecules and a plurality of ligands that binds, or is capable of binding, to biomolecules of the plurality of particle-bound biomolecules, detecting light scattered from particle-bound biomolecule-ligand complexes in the particle-bound biomolecule-ligand composition over a duration to produce a set of imaging data using the detector, and determining size or volume changes of one or more of the particle-bound biomolecule-ligand complexes during at least a portion of the duration from the set of imaging data to thereby determine the molecular binding kinetics on the particles. Related systems and computer readable media are also provided.
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Patent Agency Ranking
公开(公告)号：US20230186488A1
公开(公告)日：2023-06-15
申请号：US18062261
申请日：2022-12-06
Inventor： Shaopeng WANG , Guangzhong MA
CPC classification number: G06T7/246 , G16B15/00 , G06T2207/10056 , G06T2207/30024
Abstract: Provided herein are methods of tracking molecular dynamics in three dimensions. In some embodiments, the methods include introducing an incident light toward a second surface of a substrate to induce a plasmonic wave at least proximal to a first surface of the substrate. A population of particles is connected to the first surface of the substrate via one or more first biomolecules. In some embodiments, the methods also include detecting a change in position of the particles in the population along at least three dimensions over a duration from a change in intensity of the incident light reflected at an interface of the first surface of the substrate. Related systems and computer readable media are also provided.
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7.发明公开公开(公告)号：US20240344106A1
公开(公告)日：2024-10-17
申请号：US18751532
申请日：2024-06-24
Inventor： Shaopeng WANG , Fenni ZHANG
CPC classification number: C12Q1/06 , C12Q1/18 , G06T7/0016 , G06T7/246 , G06T2207/10056 , G06T2207/30024 , G06T2207/30241
Abstract: Provided herein are methods of assessing the presence of microbes in a liquid sample that include assessing an initial integrated scattering intensity of objects (IC0) in the sample and an integrated scattering intensity of the objects at a time t (ICt) from modified images of the liquid sample, and identifying the sample as comprising microbes for (ICt)/(IC0) above a predefined infection threshold TI. Related systems and other aspects are also provided.
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公开(公告)号：US20240019429A1
公开(公告)日：2024-01-18
申请号：US18349650
申请日：2023-07-10
Inventor： Shaopeng WANG , Guangzhong MA , Xiaoyan ZHOU
IPC: G01N33/557 , G01N33/543 , G01N21/552
CPC classification number: G01N33/557 , G01N33/54373 , G01N21/553
Abstract: Provided herein are methods of determining binding kinetics of a ligand. In some embodiments, the methods include contacting the ligand with a first surface of a substrate, which first surface comprises an electrically conductive coating and a population of receptors connected to the first surface via one or more linker moieties, wherein the receptors bind, or are capable of binding, to the ligand, applying an alternating current electric field to the substrate to induce the receptors to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the receptors over a duration. Related receptor oscillator array devices, systems and computer readable media are also provided.
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9.发明公开公开(公告)号：US20240003905A1
公开(公告)日：2024-01-04
申请号：US18212460
申请日：2023-06-21
Inventor： Shaopeng WANG , Rui WANG , Pengfei ZHANG
IPC: G01N33/68 , G01N33/543
CPC classification number: G01N33/6872 , G01N33/54386 , G01N2333/705
Abstract: Provided herein are methods of modulating temperature in detection fields and producing analyte imaging data. In some embodiments, the methods include introducing an incident light toward a second surface of a substrate to induce a plasmonic wave proximal to a first surface of the substrate such that a temperature in a selected heating space within the detection field is substantially uniformly changed to a selected temperature. Additional methods as well as related systems and computer readable media are also provided.
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10.发明公开公开(公告)号：US20230278032A1
公开(公告)日：2023-09-07
申请号：US18174541
申请日：2023-02-24
Inventor： Shaopeng WANG , Fenni ZHANG
CPC classification number: B01L3/502715 , B01L3/502761 , G06V10/14 , G06V10/25 , G06V10/44 , G06V10/62 , B01L2400/0487 , G01N2021/0106
Abstract: Disclosed herein is a system and method for quantitative detection and analysis of molecular binding kinetics of a substance with surface membrane proteins of a biological object, such as a live cell, based on detecting and tracking membrane fluctuation amplitude changes cause by membrane displacement associated with the binding of the substance with the surface membrane proteins. The molecular binding kinetics can be detected with high precision in real time from an optical image of the biological object with a differential detection method.
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