公开(公告)号：US10408757B2

公开(公告)日：2019-09-10

申请号：US15038629

申请日：2015-01-02

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Nongjian Tao ,  Hui Yu ,  Xiaonan Shan ,  Shaopeng Wang

IPC: G01N21/552 ,  C12M1/34

Abstract: Method and system to remove background noise with a differential approach in optical imaging is disclosed. The differential approach moves the sample position laterally over a small distance, and a differential image is generated from the images recorded before and after the lateral translation. This approach can significantly improve the image quality of objects, including single DNA molecules, for label-free optical imaging techniques, such as surface plasmon resonance imaging. Disclosed imaging technique provides high-resolution genome-wide restriction maps of single DNA molecules.

公开(公告)号：US20160299069A1

公开(公告)日：2016-10-13

申请号：US15038629

申请日：2015-01-02

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Nongjian Tao ,  Hui Yu ,  Xiaonan Shan ,  Shaopeng Wang

IPC: G01N21/552

CPC classification number: G01N21/553 ,  C12M41/46 ,  G01N21/552

Abstract: Method and system to remove background noise with a differential approach in optical imaging is disclosed. The differential approach moves the sample position laterally over a small distance, and a differential image is generated from the images recorded before and after the lateral translation. This approach can significantly improve the image quality of objects, including single DNA molecules, for label-free optical imaging techniques, such as surface plasmon resonance imaging. Disclosed imaging technique provides high-resolution genome-wide restriction maps of single DNA molecules.

Abstract translation: 公开了在光学成像中用差分方法去除背景噪声的方法和系统。 差分方法将样本位置横向移动一小段距离，并且从侧向平移之前和之后记录的图像产生差分图像。 这种方法可以显着提高物体（包括单个DNA分子）的图像质量，用于无标记光学成像技术，如表面等离子体共振成像。 公开的成像技术提供了单分子DNA分子的高分辨率全基因组限制性图谱。