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公开(公告)号:US09484193B2
公开(公告)日:2016-11-01
申请号:US14298911
申请日:2014-06-07
申请人: Academia Sinica
CPC分类号: H01J49/0031 , G01N33/6818 , G01N33/6851 , H01J49/0054
摘要: Apparatus and methods for automatic amino acid sequencing of a glycopeptide by mass spectrometry. The glycopeptide is fragmented by higher energy collision dissociation fragmentation, and sequentially fragmented by collision induced dissociation fragmentation. The glycopeptide Y1 ion is isolated, and the mass spectra of fragmented glycopeptide Y1 ions provide mass spectral peaks corresponding to the amino acid sequence of the glycopeptide.
摘要翻译: 通过质谱法对糖肽进行自动氨基酸测序的装置和方法。 糖肽通过较高的能量碰撞解离断裂而碎裂,并通过碰撞诱导的解离片段依次碎裂。 分离糖肽Y1离子,碎片糖肽Y1离子的质谱提供对应于糖肽的氨基酸序列的质谱峰。