Automatic amino acid sequencing of glycopeptide by Y1 ions
    1.
    发明授权
    Automatic amino acid sequencing of glycopeptide by Y1 ions 有权
    通过Y1离子自动氨基酸测序糖肽

    公开(公告)号:US09484193B2

    公开(公告)日:2016-11-01

    申请号:US14298911

    申请日:2014-06-07

    申请人: Academia Sinica

    IPC分类号: H01J49/00 G01N33/68

    摘要: Apparatus and methods for automatic amino acid sequencing of a glycopeptide by mass spectrometry. The glycopeptide is fragmented by higher energy collision dissociation fragmentation, and sequentially fragmented by collision induced dissociation fragmentation. The glycopeptide Y1 ion is isolated, and the mass spectra of fragmented glycopeptide Y1 ions provide mass spectral peaks corresponding to the amino acid sequence of the glycopeptide.

    摘要翻译: 通过质谱法对糖肽进行自动氨基酸测序的装置和方法。 糖肽通过较高的能量碰撞解离断裂而碎裂,并通过碰撞诱导的解离片段依次碎裂。 分离糖肽Y1离子,碎片糖肽Y1离子的质谱提供对应于糖肽的氨基酸序列的质谱峰。