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1.发明申请公开(公告)号：US20160168602A1
公开(公告)日：2016-06-16
申请号：US15013299
申请日：2016-02-02
Applicant: BASF SE
Inventor： Bernhard Hauer , Hans Wolfgang Höffken , Cecilia Kvarnström Branneby , Helmut Schwab , Sabine Feichtenhofer , Marlene Buchebner , Brigitte Pohn
CPC classification number: C12P7/62 , C08F8/12 , C12N9/16 , C12N9/18 , C12Y301/01001 , C08F120/18
Abstract: The invention relates to a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. According to the method, at least one polyacrylic acid ester is provided and incubated with at least one enzyme selected from enzymes (EC 3.1) acting on ester bindings, until the ester groups contained in the polyacrylic acid ester are partially or fully hydrolytically split, and optionally the modified polymer obtained thereby is isolated. The invention also relates to the enzymes used and mutants thereof, nucleic acids coding for the enzymes, vectors comprising the nucleic acids, micro-organisms comprising the vectors, and the use of the enzymes, the vectors or the micro-organisms for carrying out a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. The present application also relates to polymer reaction products that can be obtained by the method, and methods for producing esterases.
Abstract translation: 本发明涉及聚丙烯酸酯的酶催化水解方法。 根据该方法，提供至少一种聚丙烯酸酯，并与至少一种选自作用于酯结合的酶（EC 3.1）的酶进行温育，直到聚丙烯酸酯中所含的酯基部分或完全水解分裂为止，以及 由此分离获得的改性聚合物。 本发明还涉及使用的酶及其突变体，编码酶的核酸，包含核酸的载体，包含载体的微生物，以及使用酶，载体或微生物进行酶 聚丙烯酸酯酶催化水解的方法。 本申请还涉及可以通过该方法获得的聚合物反应产物和生产酯酶的方法。
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2.发明授权Method for the enzyme-catalysed hydrolysis of polyacrylic acid esters, and esterases used therefor 有权聚丙烯酸酯的酶催化水解方法和用于其的酯酶的方法公开(公告)号：US09587257B2
公开(公告)日：2017-03-07
申请号：US15013299
申请日：2016-02-02
Applicant: BASF SE
Inventor： Bernhard Hauer , Hans Wolfgang Höffken , Cecilia Kvarnström Branneby , Helmut Schwab , Sabine Feichtenhofer , Marlene Buchebner , Brigitte Pohn
CPC classification number: C12P7/62 , C08F8/12 , C12N9/16 , C12N9/18 , C12Y301/01001 , C08F120/18
Abstract: The invention relates to a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. According to the method, at least one polyacrylic acid ester is provided and incubated with at least one enzyme selected from enzymes (EC 3.1) acting on ester bindings, until the ester groups contained in the polyacrylic acid ester are partially or fully hydrolytically split, and optionally the modified polymer obtained thereby is isolated. The invention also relates to the enzymes used and mutants thereof, nucleic acids coding for the enzymes, vectors comprising the nucleic acids, micro-organisms comprising the vectors, and the use of the enzymes, the vectors or the micro-organisms for carrying out a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. The present application also relates to polymer reaction products that can be obtained by the method, and methods for producing esterases.
Abstract translation: 本发明涉及聚丙烯酸酯的酶催化水解方法。 根据该方法，提供至少一种聚丙烯酸酯，并与至少一种选自作用于酯结合的酶（EC 3.1）的酶进行温育，直到聚丙烯酸酯中所含的酯基部分或完全水解分裂为止，以及 由此分离获得的改性聚合物。 本发明还涉及使用的酶及其突变体，编码酶的核酸，包含核酸的载体，包含载体的微生物，以及使用酶，载体或微生物进行酶 聚丙烯酸酯酶催化水解的方法。 本申请还涉及可以通过该方法获得的聚合物反应产物和生产酯酶的方法。
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