公开(公告)号：US12168785B2

公开(公告)日：2024-12-17

申请号：US17270489

申请日：2018-09-03

Applicant: BGI SHENZHEN

Inventor： Lili Zhai ,  Lin Wang ,  Wenwei Zhang ,  Yuliang Dong ,  Yue Zheng ,  Fen Liu

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).