公开(公告)号：US20250066748A1

公开(公告)日：2025-02-27

申请号：US18723633

申请日：2021-12-31

Applicant: BGI Shenzhen

Inventor： Lele Wang ,  Tao Zeng ,  Zhenjun Liu ,  Junyi Chen ,  Ziyu Zhao ,  Denghui Li ,  Fei Guo ,  Zhouxiang Ji ,  Ou Wang ,  Yuxiang Li ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu

IPC: C12N9/14 ,  C12N15/70 ,  C12Q1/34 ,  C12Q1/6869

Abstract: The present invention provides a helicase BCH2X, comprising an amino acid sequence represented by any one of SEQ ID NOs: 1-3. The present invention also provides a complex structure comprising the helicase BCH2X and a binding moiety for binding polynucleotides. The present invention also provides a use of the helicase BCH2X or the complex structure comprising same in the control and characterization of polynucleotides and single-molecule nanopore sequencing.

公开(公告)号：US12168785B2

公开(公告)日：2024-12-17

申请号：US17270489

申请日：2018-09-03

Applicant: BGI SHENZHEN

Inventor： Lili Zhai ,  Lin Wang ,  Wenwei Zhang ,  Yuliang Dong ,  Yue Zheng ,  Fen Liu

IPC: C12Q1/68 ,  C12N9/12 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).

公开(公告)号：US20210340509A1

公开(公告)日：2021-11-04

申请号：US17358856

申请日：2021-06-25

Applicant: BGI SHENZHEN

Inventor： Huanhuan Liu ,  Na Guo ,  Huizhen Li ,  Zhougang Zhang ,  Hongyan Han ,  Miaomiao Guo ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu

IPC: C12N9/12 ,  C12N15/10 ,  C12Q1/6869 ,  C12N15/70 ,  C12Q1/686 ,  C12Q1/6806

Abstract: The present disclosure relates to a reverse transcriptase and an application thereof. The reverse transcriptase has mutation sites such as R450H compared with the wild-type M-MLV reverse transcriptase. The reverse transcriptase has increased polymerase activity, improved thermal stability, and reduced RNase H activity.

公开(公告)号：US20250136974A1

公开(公告)日：2025-05-01

申请号：US18725483

申请日：2021-12-31

Applicant: BGI Shenzhen

Inventor： Ou Wang ,  Xiao Shi ,  Xun Xu ,  Wenwei Zhang ,  Fei Guo ,  Siyu Bao ,  Yuliang Dong ,  Tao Zeng

IPC: C12N15/11 ,  C12N15/10 ,  C12Q1/6869

Abstract: The present invention relates to a nucleic acid molecule capable of blocking motor protein, a library containing the nucleic acid molecule, a method for constructing the nucleic acid molecule or library containing the same, and an application of the nucleic acid molecule or library containing same. The nucleic acid molecule contains at the end a modified nucleotide capable of blocking a motor protein, and the modified nucleotide is as shown in Formula I or Formula I′:

公开(公告)号：US20250066747A1

公开(公告)日：2025-02-27

申请号：US18723588

申请日：2021-12-31

Applicant: BGI Shenzhen

Inventor： Xun Xu ,  Fei Guo ,  Qiaoxia Hu ,  Denghui Li ,  Junyi Chen ,  Lele Wang ,  Ziyu Zhao ,  Zhouxiang Ji ,  Tao Zeng ,  Ou Wang ,  Yuxiang Li ,  Yuliang Dong ,  Wenwei Zhang

IPC: C12N9/14 ,  C12Q1/6869

Abstract: Provided is helicase BCH1X, which comprises an amino acid sequence represented by SEQ ID NO: 1 or 2. Further provided are a complex structure comprising helicase BCH1X and a binding moiety used for binding to a polynucleotide, and a use thereof in the control and characterization of a polynucleotide and in single molecule nanopore sequencing.

公开(公告)号：US20240392265A1

公开(公告)日：2024-11-28

申请号：US18564400

申请日：2021-09-08

Applicant: BGI SHENZHEN

Inventor： Lili Zhai ,  Qingqing Xie ,  Ruyin Cao ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu ,  Zhuo Chen

IPC: C12N9/12 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided are a thermostable B-family DNA polymerase mutant and an application thereof. The thermostable B-family DNA polymerase mutant is provided to mutate amino acid residues in at least two of three sites of group A, i.e., 408, 409, and 410 in an amino acid sequence of thermostable B-family DNA polymerases to obtain proteins having DNA polymerase activity. A-group sites (408/409/410), B-group sites (451/485), C-group sites (389/383/384), D-group sites (589/67/680), E-group sites (491/493/494/497), and F-group sites (474/478/486/480/484) of several B-family DNA polymerases are analyzed to design the mutant.

公开(公告)号：US20250066745A1

公开(公告)日：2025-02-27

申请号：US18725513

申请日：2021-12-31

Applicant: BGI SHENZHEN

Inventor： Wei Zhang ,  Yuliang Dong ,  Jing Li ,  Yue Zheng ,  Wenwei Zhang

IPC: C12N9/02 ,  C12Q1/66

Abstract: A mutant and the uses thereof. Compared with the amino acid sequence shown in SEQ ID NO: 2, the mutant has at least one of the following mutation sites: the 24th, 26th, 27th, 29th, 30th, 31st, 32nd, 33rd, 36th, 37th, 40th, 66th, 79th, 84th, 88th, 102nd, 103rd, 104th, 110th, 123rd, 124th, 138th, 152nd, 163rd, 167th, 170th, 174th, 175th, 178th, 182nd and 183rd sites.

公开(公告)号：US20240392263A1

公开(公告)日：2024-11-28

申请号：US18032755

申请日：2020-10-21

Applicant: BGI SHENZHEN

Inventor： Lili Zhai ,  Qingqing Xie ,  Ruyin Cao ,  Zi Wang ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Provided is a recombinant KOD polymerase. A KOD polymerase mutant is a protein as follows: the protein is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 48 positions in the amino acid sequence of KOD DNA polymerase GH78: 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729, without changing other amino acid sequences; and compared with KOD DNA polymerase GH78, with regard to catalysis, the recombinant DNA polymerase exhibits a faster reaction rate, better catalytic efficiency, better affinity and other advantages, thereby improving the reaction rate of DNA polymerase in sequencing and increasing the reaction read length.