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    Method of constructing sequencing library
    2.
    发明授权
    Method of constructing sequencing library 有权

    公开(公告)号：US10456769B2

    公开(公告)日：2019-10-29

    申请号：US15667841

    申请日：2017-08-03

    Applicant: BGI Shenzhen ,  BGI Shenzhen Co., Limited

    Inventor： Ou Wang ,  Xiaofang Cheng ,  Liangying Zou ,  Cankun Chang ,  Hui Jiang ,  Wenwei Zhang

    IPC: B01J19/00 ,  C12N15/10

    Abstract: Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.

    Method and reagent for constructing nucleic acid double-linker single-strand cyclical library
    3.
    发明授权
    Method and reagent for constructing nucleic acid double-linker single-strand cyclical library 有权

    公开(公告)号：US10479991B2

    公开(公告)日：2019-11-19

    申请号：US15529881

    申请日：2014-11-26

    Applicant: BGI SHENZHEN ,  BGI SHENZHEN CO., LIMITED

    Inventor： Yuan Jiang ,  Qiaoling Li ,  Andrei Alexeev ,  Evan Hurowitz ,  Xia Zhao ,  Tong Wang ,  Chao Dong ,  Dong Li ,  Radoje Drmanac ,  Wenwei Zhang ,  Hui Jiang

    IPC: C12N15/10 ,  C40B50/18 ,  B01J19/00 ,  C40B50/06

    Abstract: A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase site is provided on primer sequences and a nicking enzyme recognition sequence is either provided or not provided on same, and a first affinity tag is provided on one of the primer sequences; using USER enzyme to cleave the first product; cyclizing the cleaved first product; treating the cyclization product with either a phosphatase or a nicking enzyme; using a solid-phase vector for combination with a cyclized molecule; performing a restrictive gap translation reaction; removing by digestion any portion that did not undergo the restrictive gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and cyclizing a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments, a simplified library construction process, reduced library construction time, and reduced library construction costs.

    SEQUENCING CHIP AND PREPARATION METHOD THEREFOR
    4.
    发明申请
    SEQUENCING CHIP AND PREPARATION METHOD THEREFOR 有权

    公开(公告)号：US20230056313A1

    公开(公告)日：2023-02-23

    申请号：US18048671

    申请日：2022-10-21

    Applicant: BGI SHENZHEN

    Inventor： Zhaohui Wang ,  Handong Li ,  Yuan Li ,  Wenwei Zhang ,  Ao Chen

    IPC: C12Q1/6874 ,  H01L27/146 ,  B01L3/00

    Abstract: Provided is a sequencing chip. The sequencing chip includes: a chip main body, nucleic acids, and a phosphonic acid polymer film. The chip main body includes a plurality of chip particles arranged in a same layer, the chip particles are obtained by cutting a chip matrix along cutting lines of a wafer layer, and the chip matrix includes: the wafer layer having the cutting lines uniformly distributed thereon; a first silicon oxide layer made of silicon oxide and formed on an upper surface of the wafer layer; and a transition metal oxide layer made of a transition metal oxide and formed on an upper surface of the first silicon oxide layer. The nucleic acids are fixed on the transition metal oxide layer; and the phosphonic acid polymer film is made of a polyphosphonic acid polymer and formed on an upper surface of the transition metal oxide layer.

    SEQUENCING CHIP AND MANUFACTURING METHOD THEREFOR
    5.
    发明申请
    SEQUENCING CHIP AND MANUFACTURING METHOD THEREFOR 有权

    公开(公告)号：US20210384031A1

    公开(公告)日：2021-12-09

    申请号：US17377114

    申请日：2021-07-15

    Applicant: BGI SHENZHEN

    Inventor： Shifeng Li ,  Tengyue Li ,  Yuan Li ,  Zhaohui Wang ,  Xueqin Jiang ,  Jiacheng Chen ,  Aoli Wang ,  Fuxing Huang ,  Xiaogang Song ,  Lingling Peng ,  Handong Li ,  Wenwei Zhang

    IPC: H01L21/02 ,  H01L21/78

    Abstract: Provided are a chip matrix, a sequencing chip, and a manufacturing method thereof. The chip matrix includes: a wafer layer (111), the wafer layer (111) having cutting lines that are evenly distributed thereon; a first silicon oxide layer (112), the first silicon oxide layer (112) being made of silicon oxide and formed on an upper surface of the wafer layer (111); a transition metal oxide layer (113), the transition metal oxide layer (113) being made of transition metal oxide and formed on an upper surface of the first silicon oxide layer (112). The chip matrix has characteristics such as resistances against high temperature, high humidity and other harsh environments. Meanwhile, by changing pH, surfactant and other components of a solution containing sequences to be sequenced, a surface functional region of the chip matrix can specifically adsorb a sequence to be sequenced.

    MUTANTS AND USE THEREOF
    7.
    发明申请
    MUTANTS AND USE THEREOF 有权

    公开(公告)号：US20250066745A1

    公开(公告)日：2025-02-27

    申请号：US18725513

    申请日：2021-12-31

    Applicant: BGI SHENZHEN

    Inventor： Wei Zhang ,  Yuliang Dong ,  Jing Li ,  Yue Zheng ,  Wenwei Zhang

    IPC: C12N9/02 ,  C12Q1/66

    Abstract: A mutant and the uses thereof. Compared with the amino acid sequence shown in SEQ ID NO: 2, the mutant has at least one of the following mutation sites: the 24th, 26th, 27th, 29th, 30th, 31st, 32nd, 33rd, 36th, 37th, 40th, 66th, 79th, 84th, 88th, 102nd, 103rd, 104th, 110th, 123rd, 124th, 138th, 152nd, 163rd, 167th, 170th, 174th, 175th, 178th, 182nd and 183rd sites.

    RECOMBINANT KOD POLYMERASE
    8.
    发明申请
    RECOMBINANT KOD POLYMERASE 有权

    公开(公告)号：US20240392263A1

    公开(公告)日：2024-11-28

    申请号：US18032755

    申请日：2020-10-21

    Applicant: BGI SHENZHEN

    Inventor： Lili Zhai ,  Qingqing Xie ,  Ruyin Cao ,  Zi Wang ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu

    IPC: C12N9/12 ,  C12Q1/6869

    Abstract: Provided is a recombinant KOD polymerase. A KOD polymerase mutant is a protein as follows: the protein is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 48 positions in the amino acid sequence of KOD DNA polymerase GH78: 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729, without changing other amino acid sequences; and compared with KOD DNA polymerase GH78, with regard to catalysis, the recombinant DNA polymerase exhibits a faster reaction rate, better catalytic efficiency, better affinity and other advantages, thereby improving the reaction rate of DNA polymerase in sequencing and increasing the reaction read length.

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