公开(公告)号：US20150274808A1

公开(公告)日：2015-10-01

申请号：US14638215

申请日：2015-03-04

Applicant: BIOGEN MA INC.

Inventor： David Evans ,  R. Blake Pepinsky ,  Dingyi Wen ,  Rashmi Rohit Kshirsagar ,  Karin Lucas

IPC: C07K16/00

CPC classification number: C07K16/00 ,  C07K1/1133

Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.

Abstract translation: 本发明涉及在蛋白质如抗体中预防和消除三硫键的方法。 在一个实施方案中，作为色谱纯化方法的一部分，将蛋白质中的三硫键转化为二硫键。 在另一个实施方案中，通过在这些蛋白质的细胞培养生产期间实施本文所述的方法来抑制蛋白质中三硫键的形成。 在另一个实施方案中，通过本文所述的方法产生单克隆抗体。

公开(公告)号：US10308706B2

公开(公告)日：2019-06-04

申请号：US14638215

申请日：2015-03-04

Applicant: BIOGEN MA INC.

Inventor： David Evans ,  R. Blake Pepinsky ,  Dingyi Wen ,  Rashmi Rohit Kshirsagar ,  Karin Lucas

IPC: C07K16/00 ,  C07K1/113

Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.