公开(公告)号：US20210222244A1

公开(公告)日：2021-07-22

申请号：US17151058

申请日：2021-01-15

Applicant: Becton, Dickinson and Company

Inventor： Jody Martin ,  Mirko Corselli ,  James Ghadiali ,  Feng Ge ,  Chad Sisouvanthong

IPC: C12Q1/6876 ,  C12Q1/6841 ,  C12Q1/6837

Abstract: Systems, methods, compositions, and kits for measuring secreted factors from cells are disclosed herein, including those capable of determining single cell secretion activity and protein expression and/or gene expression simultaneously. Disclosed herein include bispecific probes comprising an anchor probe capable of specifically binding to a surface cellular target of a cell, and a capture probe capable of specifically binding to a secreted factor secreted by a cell that is associated with the capture probe. Also disclosed herein include secreted factor-binding reagents capable of specifically binding to a secreted factor bound by a capture probe, where a secreted factor-binding reagent can comprise a secreted factor-binding reagent specific oligonucleotide comprising a unique factor identifier sequence for the secreted factor-binding reagent.