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公开(公告)号:US20210222244A1
公开(公告)日:2021-07-22
申请号:US17151058
申请日:2021-01-15
Applicant: Becton, Dickinson and Company
Inventor: Jody Martin , Mirko Corselli , James Ghadiali , Feng Ge , Chad Sisouvanthong
IPC: C12Q1/6876 , C12Q1/6841 , C12Q1/6837
Abstract: Systems, methods, compositions, and kits for measuring secreted factors from cells are disclosed herein, including those capable of determining single cell secretion activity and protein expression and/or gene expression simultaneously. Disclosed herein include bispecific probes comprising an anchor probe capable of specifically binding to a surface cellular target of a cell, and a capture probe capable of specifically binding to a secreted factor secreted by a cell that is associated with the capture probe. Also disclosed herein include secreted factor-binding reagents capable of specifically binding to a secreted factor bound by a capture probe, where a secreted factor-binding reagent can comprise a secreted factor-binding reagent specific oligonucleotide comprising a unique factor identifier sequence for the secreted factor-binding reagent.