公开(公告)号：US20140024107A1

公开(公告)日：2014-01-23

申请号：US14039682

申请日：2013-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tom McCOLLUM ,  Paul PATT ,  Frank SHEN ,  Daniel Y. CHU ,  Don FLORY ,  Mike GRIFFIN ,  Xin HENG ,  Eli HEFNER

IPC: C12Q1/06

CPC classification number: C12Q1/06 ,  G01N15/1434 ,  G02B21/0008 ,  G02B21/34

Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.

Abstract translation: 将液体悬浮液中的生物细胞计数在自动细胞计数器中，其将悬浮液的图像聚焦在数字成像传感器上，该数字成像传感器包含至少4,000000个像素，每个具有2×2μm或更小的面积，并且图像的场 视图至少3 mm2。 该传感器使得计数器能够将光学部件压缩成高度小于20cm的光路，当垂直布置时，整个光路的方向没有变化，并且整个仪器的占地面积小于300cm 2。 光源的激活，传感器图像的自动聚焦和数字单元计数都是通过将样品架简单插入到仪器中来启动的。 将悬浮液置于样品室中，其形状为成形以确保细胞计数器中载玻片的正确取向。