公开(公告)号：US20230194517A1

公开(公告)日：2023-06-22

申请号：US18083131

申请日：2022-12-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Eli HEFNER

IPC: G01N33/543 ,  G01N21/64 ,  B01L3/00

CPC classification number: G01N33/54386 ,  G01N21/6428 ,  G01N21/6458 ,  B01L3/502761 ,  G01N2021/6439 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2200/0652

Abstract: Systems and methods for multiplex detection through measurement of localized fluorescence ratios are disclosed herein. This can include creating a plurality of capture structures that each include a detection portion that can couple with a target analyte and a stem that can include a capture structure code uniquely identifying a type of the capture structure. The capture structures can be attached to a sample surface and mixed with a sample containing a plurality of target analytes. A location and the capture structure code of each of the capture structures can be determined. A location at which a target analyte is bound to one of the capture structures can be identified, and the target analyte can be determined based on the capture structure code of the capture structure at the location at which the target analyte is bound to one of the capture structures.

公开(公告)号：US20200209229A1

公开(公告)日：2020-07-02

申请号：US16722706

申请日：2019-12-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： William STRONG ,  Eli HEFNER

IPC: G01N33/543 ,  G01N33/58 ,  C12Q1/6869 ,  C12Q1/6834

Abstract: Described are methods and compositions for sequential multiplex detection of target analytes in a sample. The method comprises contacting the sample comprising analytes immobilized on a solid support with binding agents that specifically bind an analyte in the sample, wherein each of the binding agents binds a different analyte and is attached to a single-stranded nucleic acid molecule comprising a unique sequence. The sample is then contacted with a labeled complementary nucleic acid molecule that binds the single-stranded nucleic acid molecule attached to one binding agent. The signal from the label is detected, and then reduced or eliminated. The sample can be simultaneously contacted with a second labeled complementary nucleic acid molecule that binds a different binding agent, and the signal from the second label is detected. The process is repeated for each additional analyte in the sample, thereby sequentially detecting the presence of the analytes in the sample.

公开(公告)号：US20190284610A1

公开(公告)日：2019-09-19

申请号：US16353865

申请日：2019-03-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Eli HEFNER ,  Yann JOUVENOT

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/70

Abstract: Compositions, kits, and methods are provided for the normalization of a quantitative polymerase chain reaction (PCR) amplification. Also provided are compositions, kits, and methods for multiplexing qPCR amplification of two or more target nucleic acids in the same well.

公开(公告)号：US20230349892A1

公开(公告)日：2023-11-02

申请号：US18139829

申请日：2023-04-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： William STRONG ,  Eli HEFNER ,  Carl MARLOWE

IPC: G01N33/543 ,  G01N33/58

CPC classification number: G01N33/543 ,  G01N33/582 ,  G01N2458/10

Abstract: The disclosure provides a highly sensitive assay to detect the presence of a target analyte in a sample.

公开(公告)号：US20140024107A1

公开(公告)日：2014-01-23

申请号：US14039682

申请日：2013-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tom McCOLLUM ,  Paul PATT ,  Frank SHEN ,  Daniel Y. CHU ,  Don FLORY ,  Mike GRIFFIN ,  Xin HENG ,  Eli HEFNER

IPC: C12Q1/06

CPC classification number: C12Q1/06 ,  G01N15/1434 ,  G02B21/0008 ,  G02B21/34

Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.

Abstract translation: 将液体悬浮液中的生物细胞计数在自动细胞计数器中，其将悬浮液的图像聚焦在数字成像传感器上，该数字成像传感器包含至少4,000000个像素，每个具有2×2μm或更小的面积，并且图像的场 视图至少3 mm2。 该传感器使得计数器能够将光学部件压缩成高度小于20cm的光路，当垂直布置时，整个光路的方向没有变化，并且整个仪器的占地面积小于300cm 2。 光源的激活，传感器图像的自动聚焦和数字单元计数都是通过将样品架简单插入到仪器中来启动的。 将悬浮液置于样品室中，其形状为成形以确保细胞计数器中载玻片的正确取向。