公开(公告)号：US20220259644A1

公开(公告)日：2022-08-18

申请号：US17728540

申请日：2022-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

IPC: C12Q1/6841 ,  G01N33/574 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N33/487

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

公开(公告)号：US10975422B2

公开(公告)日：2021-04-13

申请号：US16049057

申请日：2018-07-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

IPC: C12Q1/68 ,  G01N33/487 ,  G01N33/574 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N27/447

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

公开(公告)号：US12222345B2

公开(公告)日：2025-02-11

申请号：US15931928

申请日：2020-05-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Christopher Siemer ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Gene Parunak ,  Aaron Kehrer ,  Jon Meines

IPC: G01N33/483 ,  G01N21/25 ,  G01N21/64 ,  G01N21/76 ,  G01N33/487 ,  G02B21/06 ,  G02B21/26 ,  A61B5/00

Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

公开(公告)号：US11345951B2

公开(公告)日：2022-05-31

申请号：US16048975

申请日：2018-07-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

IPC: C12Q1/68 ,  C12Q1/6841 ,  G01N33/574 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N33/487 ,  G01N27/447

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

公开(公告)号：US11199532B2

公开(公告)日：2021-12-14

申请号：US17323176

申请日：2021-05-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Christopher Siemer ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Gene Parunak ,  Aaron Kehrer ,  Jon Meines

IPC: G01N21/64 ,  G01N21/25 ,  G02B21/06 ,  G02B21/26 ,  A61B5/00 ,  G01N21/76 ,  G01N33/483 ,  G01N33/487 ,  G01N33/48

Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

公开(公告)号：US20200271637A1

公开(公告)日：2020-08-27

申请号：US15931928

申请日：2020-05-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Christopher Siemer ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Gene Parunak ,  Aaron Kehrer ,  Jon Meines

IPC: G01N33/483 ,  G01N21/64

Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

公开(公告)号：US20220291190A1

公开(公告)日：2022-09-15

申请号：US17830325

申请日：2022-06-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Christopher Siemer ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Gene Parunak ,  Aaron Kehrer ,  Jon Meines

IPC: G01N33/483 ,  G02B21/26 ,  G02B21/06 ,  G01N21/25 ,  G01N21/76 ,  G01N33/487 ,  G01N21/64

Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

公开(公告)号：US20210270800A1

公开(公告)日：2021-09-02

申请号：US17323176

申请日：2021-05-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Christopher Siemer ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Gene Parunak ,  Aaron Kehrer ,  Jon Meines

IPC: G01N33/483 ,  G01N21/64

Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.