公开(公告)号：US11345951B2

公开(公告)日：2022-05-31

申请号：US16048975

申请日：2018-07-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

IPC: C12Q1/68 ,  C12Q1/6841 ,  G01N33/574 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N33/487 ,  G01N27/447

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

公开(公告)号：US20210040567A1

公开(公告)日：2021-02-11

申请号：US17077095

申请日：2020-10-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Yi Zhou ,  Saedeh Sepehri ,  Christopher Siemer

IPC: C12Q1/6886 ,  C12Q1/6841 ,  G01N33/574 ,  B01L3/00 ,  C12Q1/6834

Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.

公开(公告)号：US20220259644A1

公开(公告)日：2022-08-18

申请号：US17728540

申请日：2022-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

IPC: C12Q1/6841 ,  G01N33/574 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N33/487

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

公开(公告)号：US12030051B2

公开(公告)日：2024-07-09

申请号：US16419485

申请日：2019-05-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Austin Payne ,  Priyadarshini Gogoi ,  Christopher Siemer ,  Yi Zhou ,  Saedeh Javdani Sepehri

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/527 ,  B01L3/5635 ,  B01L7/00 ,  G01N35/0098 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0684 ,  B01L2300/06 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.

公开(公告)号：US20220251668A1

公开(公告)日：2022-08-11

申请号：US17728789

申请日：2022-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Yi Zhou ,  Saedeh Sepehri ,  Christopher Siemer

IPC: C12Q1/6886 ,  C12Q1/6841 ,  G01N33/574 ,  B01L3/00 ,  C12Q1/6834

Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.

公开(公告)号：US10975422B2

公开(公告)日：2021-04-13

申请号：US16049057

申请日：2018-07-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

IPC: C12Q1/68 ,  G01N33/487 ,  G01N33/574 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N27/447

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

公开(公告)号：US20240307880A1

公开(公告)日：2024-09-19

申请号：US18674938

申请日：2024-05-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Kyle Gleason ,  Austin Payne ,  Priyadarshini Gogoi ,  Christopher Siemer ,  Yi Zhou ,  Saedeh Javdani Sepehri

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/527 ,  B01L3/5635 ,  B01L7/00 ,  G01N35/0098 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0684 ,  B01L2300/06 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/0864 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.