公开(公告)号：US20240384335A1

公开(公告)日：2024-11-21

申请号：US18666949

申请日：2024-05-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Houser ,  Andrew M. Prantner

IPC: C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/686

Abstract: The invention relates to methods for identifying, determining and quantifying linkages in fragments of nucleic acids.

公开(公告)号：US20140067277A1

公开(公告)日：2014-03-06

申请号：US14075879

申请日：2013-11-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Houser

IPC: G06F19/18

CPC classification number: G06F19/18 ,  C12Q1/6858 ,  G06F19/22 ,  G06F19/24 ,  C12Q2527/107 ,  C12Q2537/165

Abstract: Systems, methods and apparatus for an automated analysis of a collection of melt curves is provided. The analysis can identify certain characteristics of double stranded nucleotide sequences (e.g. DNA or other nucleotide sequences) which were melted. For example, a variation (e.g. a mutation) in the sequences (also called amplicons) may be determined from the analysis. The amplicons may be amplified via any amplification mechanism, such as PCR or Ligase chain reaction (LCR). The automated analysis can include identifying a melt region, normalizing a melt curve, and clustering melt curves.

Abstract translation: 提供了用于自动分析熔体曲线集合的系统，方法和装置。 分析可以鉴定被熔化的双链核苷酸序列（例如DNA或其他核苷酸序列）的某些特征。 例如，可以从分析中确定序列（也称为扩增子）中的变异（例如突变）。 扩增子可以通过任何扩增机制扩增，如PCR或连接酶链反应（LCR）。 自动化分析可以包括识别熔体区域，使融化曲线标准化和聚集熔体曲线。

公开(公告)号：US09864832B2

公开(公告)日：2018-01-09

申请号：US14075879

申请日：2013-11-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Houser

IPC: G01N33/53 ,  G06F19/18 ,  C12Q1/68 ,  G06F19/24 ,  G06F19/22

CPC classification number: G06F19/18 ,  C12Q1/6858 ,  G06F19/22 ,  G06F19/24 ,  C12Q2527/107 ,  C12Q2537/165

Abstract: Systems, methods and apparatus for an automated analysis of a collection of melt curves is provided. The analysis can identify certain characteristics of double stranded nucleotide sequences (e.g. DNA or other nucleotide sequences) which were melted. For example, a variation (e.g. a mutation) in the sequences (also called amplicons) may be determined from the analysis. The amplicons may be amplified via any amplification mechanism, such as PCR or Ligase chain reaction (LCR). The automated analysis can include identifying a melt region, normalizing a melt curve, and clustering melt curves.