公开(公告)号：US20240318239A1

公开(公告)日：2024-09-26

申请号：US18733879

申请日：2024-06-05

申请人： NANJING PREGENE BIOTECHNOLOGY CO., LTD

发明人： Wu SHANG ,  Youxiang WANG ,  Zhijie YANG ,  Yiliang ZHANG ,  Zhengxin JIANG

IPC分类号： C12Q1/6858

CPC分类号： C12Q1/6858

摘要： This application discloses a digital amplification detection method for simultaneously identifying multiple genotypes, a detection product and a detection kit, and relates to the technical field of gene detection. The detection method includes: S1, extracting sample DNA; and preparing a primer-probe mixed solution, including: synthesizing primers and probes, where for each target gene, at least two probes that bind to the target gene are designed; and modifying the primers with a small molecule compound and a nucleotide sequence capable of forming a secondary structure to obtain the primer-probe mixed solution; S2, carrying out a PCR reaction; and S3, analyzing detection data to obtain genotypes at different loci.

公开(公告)号：US20240218463A1

公开(公告)日：2024-07-04

申请号：US18418415

申请日：2024-01-22

申请人： XIZANG VOCATIONAL TECHNICAL COLLEGE

发明人： MANJUN YANG ,  CAIXIA ZHANG ,  YISHUI CAI

IPC分类号： C12Q1/6895 ,  C12Q1/6806 ,  C12Q1/6858 ,  C12Q1/686 ,  G01N27/447

CPC分类号： C12Q1/6895 ,  C12Q1/6806 ,  C12Q1/6858 ,  C12Q1/686 ,  G01N27/44726 ,  C12Q2600/13

摘要： The present invention discloses a DNA barcode for screening Floccularia luteovirens with high total polysaccharide content, belonging to the technical field of screening of edible fungi germplasm resources. Compared with a traditional breeding method and other existing DNA barcode technologies, the present invention has the advantages of time saving, labor saving, money saving, accuracy and high efficiency, plays a positive role in genetic breeding of high-quality Floccularia luteovirens, and also provides an effective method for identification and protection of germplasm resources.

公开(公告)号：US20240188520A1

公开(公告)日：2024-06-13

申请号：US18436876

申请日：2024-02-08

申请人： YUNNAN NORMAL UNIVERSITY

发明人： Ling MA ,  Yi SHANG ,  Sanwen HUANG ,  Chunzhi ZHANG ,  Dongli GAO

IPC分类号： C12Q1/6809 ,  C07K14/415 ,  C12Q1/6858 ,  C12Q1/6895

CPC分类号： C12Q1/6809 ,  C07K14/415 ,  C12Q1/6858 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/158

摘要： The present application relates to the technical field of genetic breeding, and provides a method for identifying whether a diploid potato is self-compatible. The method relates to identifying whether a StSCI gene in the diploid potato is transcribed and expressed. Also disclosed is a method for identifying whether a StSCI gene is expressed by using molecular marker, and a method of screening for the molecular marker, which includes: obtaining the genome sequence information of parental materials, screening for difference sites of the parental materials, screening for the molecular marker, and identifying whether the screened molecular marker are usable. As for the identification of the self-compatibility of a diploid potato by using the screened molecular marker, the identification workload is small, a lot of time is saved, and the identification result is not affected by the environment, and it is accurate and reliable.

公开(公告)号：US20240182982A1

公开(公告)日：2024-06-06

申请号：US18523606

申请日：2023-11-29

申请人： Centre for Novostics

发明人： Yuk-Ming Dennis Lo ,  Kwan Chee Chan ,  Peiyong Jiang ,  Lizhen Ma ,  Ze Zhou

IPC分类号： C12Q1/6886 ,  C12Q1/6858

CPC分类号： C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/112 ,  C12Q2600/154 ,  C12Q2600/156

摘要： Fragmentomic features provide various properties of the sample (e.g., urine or plasma) and/or of a subject. Relative contributions or enrichment of clinically-relevant DNA (e.g., type(s) of transrenal and non-transrenal urinary cfDNA) are provided using fragmentomic features of urinary cell-free DNA. Such measurements could be used for reflecting the glomerular permeability and monitoring various diseases, e.g., kidney abnormality. The fragmentomic features can include a corrected urinary DNA concentration, size, end motifs of urinary DNA molecules, and cfDNA molecules from open chromatin regions (OCR) of one or more tissues. In addition, nuclease activities or other fragmentation processes of cfDNA are determined based on relative contributions concerning the different profiles of cfDNA cleavage, which are also used for determining contribution of cfDNA from tissue(s), level of pathology, and gestational age.

公开(公告)号：US20240182969A1

公开(公告)日：2024-06-06

申请号：US18420290

申请日：2024-01-23

申请人： Avellino Lab USA, Inc.

发明人： Larry DEDIONISIO

IPC分类号： C12Q1/6883 ,  C12Q1/6858

CPC分类号： C12Q1/6883 ,  C12Q1/6858 ,  C12Q2600/156

摘要： Methods for treating keratoconus in a subject based on diagnosing or prognosing of keratoconus in the subject or predicting risk of keratoconus in the subject in accordance with detecting genetic variants in a sample from the subject are described.

公开(公告)号：US11987836B2

公开(公告)日：2024-05-21

申请号：US15510331

申请日：2015-09-17

申请人： Global Life Sciences Solutions USA LLC

发明人： Ryan Charles Heller ,  Nichole Lea Wood ,  Robert Scott Duthie ,  John Richard Nelson ,  Wei Gao ,  Michael James Rishel ,  Klaus Gustav Hentrich

IPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6858 ,  C12Q1/6869

CPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q2521/501 ,  C12Q2531/125

摘要： A method is provided for generating single-stranded DNA circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable of template-independent, intramolecular ligation of single-stranded DNA to generate the single-stranded DNA circles. All the steps of the method are performed without any intermediate nucleic acid isolation or nucleic acid purification. The single-stranded DNA circles may be amplified and further analyzed. Also provided is a kit which comprises compositions for carrying out the novel methods.

公开(公告)号：US20240158844A1

公开(公告)日：2024-05-16

申请号：US18179192

申请日：2023-03-06

申请人： Fuzhou Maixin Biotech Co., Ltd. China

发明人： Yiwei HUANG ,  Chun MENG ,  Jing HONG ,  Wenxiao MA ,  Xiaoya WANG ,  Qixin LIN

IPC分类号： C12Q1/6858 ,  C12Q1/6818

CPC分类号： C12Q1/6858 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q2600/156

摘要： A dual-probe method for fluorescence quantitative PCR (Polymerase Chain Reaction) is disclosed. The method involves the use of a Taqman dual-probe detection system with the same pair of primers. The dual probes includes a detection probe and a reference probe. The detection probe targets the wild-type sequence at the hotspot mutation site, which can cover multiple adjacent mutations. The reference probe targets the wild-type sequence adjacent to the target sequence. Both probes share the same pair of upstream and downstream primers. This method uses a single reaction qPCR (quantitative PCR) method, which can simultaneously detect multiple adjacent mutations. It effectively saves tissue samples and greatly shortens the testing period. Moreover, the close proximity of the target and reference sequences enhances the reliability and accuracy of the results, making it widely applicable in various fields.

公开(公告)号：US20240158842A1

公开(公告)日：2024-05-16

申请号：US18282017

申请日：2022-03-15

申请人： COUNTAGEN AB

发明人： Felix NEUMANN ,  Leiore Ajuria ASTOBIZA ,  Joost BERGMAN

IPC分类号： C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/6876

CPC分类号： C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/6876

摘要： The invention relates to methods and uses for determining the efficiency of a genetic editing procedure comprising the steps of: (i) providing a sample from a genetic-editing procedure, the sample comprising one or more correctly-edited polynucleotide sequence and/or one or more unedited polynucleotide sequence; (ii) performing Rolling Circle Amplification, to generate RCA-Products from the one or more polynucleotide sequences in the sample; and (iii) determining the efficiency of the genetic-editing procedure based on the presence of the RCA-Products generated in step (ii).

公开(公告)号：US11976319B2

公开(公告)日：2024-05-07

申请号：US17359247

申请日：2021-06-25

申请人： Pacific Biosciences of California, Inc.

发明人： Joan Wilson

IPC分类号： C12Q1/6855 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6858 ,  C12Q1/686

CPC分类号： C12Q1/6809 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/68 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2531/107 ,  C12Q1/6806 ,  C12Q2521/101 ,  C12Q2521/501 ,  C12Q2525/191 ,  C12Q2525/301

摘要： The present disclosure provides improved methods for generating asymmetrically-tagged nucleic acid constructs, compositions comprising such constructs, and kits and systems for generating such constructs.

公开(公告)号：US20240084366A1

公开(公告)日：2024-03-14

申请号：US18516576

申请日：2023-11-21

申请人： Prognosys Biosciences, Inc.

发明人： Mark S. Chee

IPC分类号： C12Q1/6837 ,  B01L3/00 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C40B30/04 ,  C40B60/04 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68

CPC分类号： C12Q1/6837 ,  B01L3/502715 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C40B30/04 ,  C40B60/04 ,  G01N33/5308 ,  G01N33/543 ,  G01N33/54366 ,  G01N33/6845 ,  G01N33/6848 ,  C12Q1/6858 ,  G01N2458/10

摘要： The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.