公开(公告)号：US5876947A

公开(公告)日：1999-03-02

申请号：US900660

申请日：1997-07-25

申请人： Bohdan J. Kudryk ,  Colvin M. Redman ,  Jian-Zhong Zhang

发明人： Bohdan J. Kudryk ,  Colvin M. Redman ,  Jian-Zhong Zhang

IPC分类号： C07K16/18 ,  C12P21/08 ,  G01N33/86 ,  G01N33/53 ,  C07K16/36 ,  G01N33/536 ,  G01N33/543

CPC分类号： C07K16/18 ,  G01N33/86 ,  Y10S435/972 ,  Y10S435/975

摘要： The invention provides monospecific antibodies that are specifically reactive with fibrinopeptide B (FPB) and with fibrinogen and fragments thereof containing the amino acid sequence defined by SEQ ID NO:1. The invention also provides anti-FPB probes, including monospecific anti-FPB antibodies that have been detectably labeled. In addition, the invention provides methods of using the monospecific antibodies for detection of fibrinopeptide B, as well as reagents and kits for performing the methods. For example, the invention provides a method for detecting fibrinopeptide B with specificity in biological samples such as blood samples, by using the antibody to immunometrically bind to the fibrinopeptide B. Diagnostic methods for determining information associated with atherogenesis and/or thrombogenesis. The invention further provides continuous cell lines (hybridomas) that produce monospecific anti-FPB antibodies.

摘要翻译： 本发明提供了与纤维蛋白肽B（FPB）特异性反应的单特异性抗体以及含有由SEQ ID NO：1定义的氨基酸序列的纤维蛋白原及其片段。 本发明还提供抗FPB探针，包括可检测标记的单特异性抗FPB抗体。 此外，本发明提供了使用单特异性抗体检测纤维蛋白肽B的方法，以及用于进行该方法的试剂和试剂盒。 例如，本发明提供了通过使用免疫结合纤维蛋白肽B的抗体来检测生物样品例如血液样品中特异性的纤维蛋白肽B的方法。用于确定与动脉粥样化形成和/或血栓形成相关的信息的诊断方法。 本发明还提供产生单特异性抗FPB抗体的连续细胞系（杂交瘤）。