公开(公告)号：US11856970B2

公开(公告)日：2024-01-02

申请号：US16471298

申请日：2019-01-18

Applicant: CHR. HANSEN A/S

Inventor： Dorthe Sandvang ,  Ole Knudsmark Sjoeholm ,  Line Skjoet-Rasmussen ,  Jette Mundus Nielsen

IPC: A23K50/40 ,  A01N63/22 ,  A23K10/18 ,  C12N1/20 ,  C12R1/125

CPC classification number: A23K50/40 ,  A01N63/22 ,  A23K10/18 ,  C12N1/205 ,  C12R2001/125

Abstract: Described are the Bacillus subtilis strain deposited as DSM 32685 and mutant strains thereof which are susceptible to relevant antibiotics, have inhibitory activity against, for example, Staphylococcus, Streptococcus, E. coli and Salmonella, and have the ability to facilitate degradation of non-starch polysaccharides and thereby increase the amount of available oligosaccharides (sugar) in animal feed. Also described are compositions comprising at least one Bacillus subtilis strain of the disclosure and optionally further bacteria and/or one or more types of yeast and methods of using such compositions.

公开(公告)号：US11291695B2

公开(公告)日：2022-04-05

申请号：US16492897

申请日：2018-03-14

Applicant: CHR. HANSEN A/S

Inventor： Dorthe Sandvang ,  Tina Styrishave

IPC: A23K1/17 ,  A23K1/165 ,  A23K1/18 ,  A61K47/00 ,  A61K45/00 ,  A01N63/00 ,  A61K39/108 ,  A61K39/08 ,  A61K35/742 ,  A23K50/75 ,  A23K10/18 ,  A61K9/00 ,  C12N1/20 ,  C12R1/125

Abstract: The present invention provides a Bacillus subtilis strain selected from the group consisting of a) the strain deposited as DSM32324, b) the strain deposited as DSM32325, and c) a mutant strain of (a) or (b) which has sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol; and has inhibitory activity against E. coli and Clostridium perfringens. The invention further relates to Bacillus compositions comprising at least one Bacillus subtilis strain of the invention, preferably the Bacillus subtilis strain DSM32324 and/or the Bacillus subtilis strain DSM32325, as Direct Fed Microbial (DFM), premix, animal feed additive or animal feed. The invention provides a method of improving one or more animal performance parameters selected from the group consisting of i) increased weight gain (WG), ii) lower feed conversion ratio (FCR), iii) lower necrotic enteritis lesion scoring, iv) lower necrotic enteritis frequency, v) lower necrotic enteritis mortality, vi) increased European Production Efficacy Factor (EPEF), and vii) lower mortality, by feeding a strain or a composition according to the invention to an animal.