公开(公告)号：US20230159958A1

公开(公告)日：2023-05-25

申请号：US17995571

申请日：2021-04-06

Applicant: CYTIVA SWEDEN AB

Inventor： Andreas Jonsson ,  Daniel Ivansson

IPC: C12N15/90 ,  C12N9/12 ,  C12N15/85

CPC classification number: C12N15/907 ,  C12N9/12 ,  C12N15/85 ,  C12N2800/30 ,  C12N2800/106

Abstract: The present disclosure relates to a method for targeted integration of a donor vector into a specific pre-defined genomic location of an isolated eukaryotic host cell. The vector and host cell together comprise nucleic acid components allowing for the selection of cells having integrated the donor vector into the pre-defined genomic location of the host cell. In addition, it provides for the identification of any random integrations of the donor vector(s) into other parts of the host cell genome. Once identified, such cells present an excellent alternative for subsequent recombinant protein production.