公开(公告)号：US20230159958A1

公开(公告)日：2023-05-25

申请号：US17995571

申请日：2021-04-06

Applicant: CYTIVA SWEDEN AB

Inventor： Andreas Jonsson ,  Daniel Ivansson

IPC: C12N15/90 ,  C12N9/12 ,  C12N15/85

CPC classification number: C12N15/907 ,  C12N9/12 ,  C12N15/85 ,  C12N2800/30 ,  C12N2800/106

Abstract: The present disclosure relates to a method for targeted integration of a donor vector into a specific pre-defined genomic location of an isolated eukaryotic host cell. The vector and host cell together comprise nucleic acid components allowing for the selection of cells having integrated the donor vector into the pre-defined genomic location of the host cell. In addition, it provides for the identification of any random integrations of the donor vector(s) into other parts of the host cell genome. Once identified, such cells present an excellent alternative for subsequent recombinant protein production.

公开(公告)号：US20240043879A1

公开(公告)日：2024-02-08

申请号：US18467658

申请日：2023-09-14

Applicant: Cytiva Sweden AB

Inventor： Daniel Ivansson ,  Andreas Castan

IPC: C12N15/90 ,  C12P21/00 ,  C07K16/00

CPC classification number: C12N15/907 ,  C12P21/00 ,  C07K16/00 ,  C12N2510/02

Abstract: The present invention relates to an improved method for cell line development (CLD) which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells.
The method combines site-directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI) and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts using SDI from that point on.