利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

2 条记录 (耗时 0.015 秒)

    METHOD OF DESIGNING ADDRESSABLE ARRAY SUITABLE FOR DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING LIGASE DETECTION REACTION
    1.
    发明申请
    METHOD OF DESIGNING ADDRESSABLE ARRAY SUITABLE FOR DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING LIGASE DETECTION REACTION 有权
    设计适用于检测核酸序列差异的可寻址阵列的方法使用LIGASE检测反应

    公开(公告)号：US20160215329A1

    公开(公告)日：2016-07-28

    申请号：US15096446

    申请日：2016-04-12

    申请人： Cornell Research Foundation, Inc.

    发明人： Francis BARANY ,  Monib ZIRVI ,  Norman P. GERRY ,  Reyna FAVIS ,  Richard KLIMAN

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2527/107 ,  C12Q2521/501 ,  C12Q2521/319 ,  C12Q2565/501

    摘要： The present invention is directed to a method of designing a plurality of capture oligonucleotide probes for use on a support to which complementary oligonucleotide probes will hybridize with little mismatch, where the plural capture oligonucleotide probes have melting temperatures within a narrow range. The present invention further relates to an oligonucleotide array comprising of a support with the plurality of oligonucleotide probes immobilized on the support, a method of using the support to detect single-base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences, and a kit for such detection, which includes the support on which the oligonucleotides have been immobilized.

    摘要翻译： 本发明涉及一种设计多个捕获寡核苷酸探针的方法，所述多个捕获寡核苷酸探针用于互补寡核苷酸探针与少量错配杂交的载体上，其中多个捕获寡核苷酸探针具有在窄范围内的熔融温度。 本发明还涉及包含固定在载体上的多个寡核苷酸探针的载体的寡核苷酸阵列，使用载体检测多个靶核苷酸序列中的单碱基变化，插入，缺失或易位的方法 和用于这种检测的试剂盒，其包括寡核苷酸已被固定的载体。