公开(公告)号：US11827620B2

公开(公告)日：2023-11-28

申请号：US16340711

申请日：2017-10-12

申请人： UNIVERSITE DE GENEVE

发明人： Gianpaolo Rando ,  Nicolas Winssinger ,  Eric Lindberg ,  Marcello Anzola

IPC分类号： C07D401/12 ,  C07C205/56 ,  C12Q1/6816 ,  C07C205/57 ,  C08L71/02 ,  C08L77/00 ,  C07F9/655 ,  C09B29/08 ,  G01N21/00 ,  G01N21/62 ,  G01N21/63 ,  G01N21/64

CPC分类号： C07D401/12 ,  C07C205/56 ,  C07C205/57 ,  C12Q1/6816 ,  C07F9/65522 ,  C08L71/02 ,  C08L77/00 ,  C09B29/0813 ,  G01N21/00 ,  G01N21/62 ,  G01N21/63 ,  G01N21/64 ,  G01N21/6428 ,  C12Q1/6816 ,  C12Q2565/113 ,  C12Q2565/501 ,  C12Q2563/107

摘要： The present invention relates to new profluorophores and conjugates thereof and their use for the detection of target molecule in a sample, in particular nucleic acid target molecules. The invention relates to new profluorophores and new fluorophores and methods of use thereof particularly useful in the fields of diagnostics and quality control.

公开(公告)号：US20230258634A1

公开(公告)日：2023-08-17

申请号：US17944439

申请日：2022-09-14

申请人： KELLBENX INCORPORATED

发明人： Hassan BENNANI ,  Leonard H. KELLNER

IPC分类号： G01N33/569 ,  C12Q1/6883 ,  G01N33/68 ,  G01N33/80 ,  C12Q1/6806 ,  C12N5/00 ,  C12N5/078

CPC分类号： G01N33/56966 ,  C12Q1/6883 ,  G01N33/6893 ,  G01N33/80 ,  C12Q1/6806 ,  C12N5/0087 ,  C12N5/0641 ,  C12Q2600/156 ,  G01N2800/385 ,  G01N2800/387 ,  C12Q2531/113 ,  C12Q2565/501 ,  C12Q2600/158

摘要： The disclosure relates to methods of preparation of fetal nucleated red blood cells (NRBCs) from biological samples for diagnostic testing.

公开(公告)号：US20190242884A1

公开(公告)日：2019-08-08

申请号：US16386087

申请日：2019-04-16

申请人： Plexbio Co., Ltd.

发明人： Dean TSAO ,  Chin-Shiou HUANG ,  Yao-Kuang CHUNG

IPC分类号： G01N33/545 ,  C12Q1/6813 ,  G01N33/553 ,  G01N33/543 ,  C12Q1/6834 ,  C12Q1/6816

CPC分类号： G01N33/545 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6834 ,  G01N33/54326 ,  G01N33/54393 ,  G01N33/553 ,  C12Q2563/149 ,  C12Q2565/501

摘要： Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and include a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.

公开(公告)号：US20190085014A1

公开(公告)日：2019-03-21

申请号：US16149114

申请日：2018-10-01

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6876 ,  C07H19/10 ,  C12Q1/68 ,  C12Q1/6874 ,  C40B40/00

CPC分类号： C12Q1/6869 ,  C07B2200/11 ,  C07H19/10 ,  C07H19/14 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2525/186 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/501 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20190031705A1

公开(公告)日：2019-01-31

申请号：US16150185

申请日：2018-10-02

申请人： The Trustees of Columbia University in the City of New York

发明人： Jingyue Ju ,  Zengmin Li ,  John Robert Edwards ,  Yasuhiro Itagaki

IPC分类号： C07H19/14 ,  C12Q1/6869 ,  C07H19/10 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6872 ,  C12Q1/68 ,  C12Q1/686

CPC分类号： C12Q1/6869 ,  C07B2200/11 ,  C07H19/10 ,  C07H19/14 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/117 ,  C12Q2525/186 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/501 ,  C40B40/00

摘要： This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.

公开(公告)号：US20180126354A1

公开(公告)日：2018-05-10

申请号：US15804924

申请日：2017-11-06

申请人： Washington University ,  Multiple Myeloma Research Foundation

发明人： Brian White ,  Irena Lanc ,  Robert Fulton ,  Daniel Auclair ,  Michael H. Tomasson

IPC分类号： B01J19/00 ,  C40B40/06 ,  C12Q1/68 ,  G06F19/22 ,  C12P19/34

CPC分类号： B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00722 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q2600/156 ,  C40B40/06 ,  G16B20/00 ,  G16B30/00 ,  C12Q2535/122 ,  C12Q2563/131 ,  C12Q2565/501

摘要： A sequencing capture array for identifying mutations in Multiple Myeloma is disclosed. Also disclosed are targeted next generation sequencing methods for identifying SNV, CNV, and translocation mutations in Multiple Myeloma tumor cells. A capture array representing fewer than 500 genes implicated in Multiple Myeloma can be used to analyze tumor mutations and create a personalized treatment plan for a Multiple Myeloma patient. Analytical methods are presented that allow tumor mutations to be elucidated with coverage at a sequencing depth of no more than 500×, or as low as 100×, with optimal efficiency achieved at a sequencing depth of about 300×.

公开(公告)号：US20180057869A1

公开(公告)日：2018-03-01

申请号：US15697617

申请日：2017-09-07

申请人： SEEGENE, INC.

发明人： Jong Yoon CHUN ,  Young Jo LEE

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6851 ,  C12Q2527/107 ,  C12Q2561/109 ,  C12Q2565/1015 ,  C12Q2561/113 ,  C12Q2525/161 ,  C12Q2561/101 ,  C12Q2521/319 ,  C12Q2525/121 ,  C12Q2537/149 ,  C12Q2565/101 ,  C12Q2565/501

摘要： The present invention relates to the detection of a target nucleic acid sequence by a PCE-NH (PTO Cleavage and Extension-Dependent Non-Hybridization) assay. The present invention adopts the occurrence of the inhibition of the hybridization between the HO with the CTO by the formation of the target-dependent extended duplex. Therefore, the present invention may detect target sequences even when the HO is not cleaved. In this regard, the design of the 5′-tagging portion of PTO, CTO and HO sequences may be readily performed and the conditions for reactions may be also easily established. In addition, the detection of the hybrid between the CTO and the HO may be performed in a different vessel from that for the extension of the CTO.

公开(公告)号：US20180002744A1

公开(公告)日：2018-01-04

申请号：US15644273

申请日：2017-07-07

申请人： Cornell Research Foundation, Inc.

发明人： Francis BARANY ,  Monib ZIRVI ,  Norman P. GERRY ,  Reyna FAVIS ,  Richard KLIMAN

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2527/107 ,  C12Q2521/501 ,  C12Q2521/319 ,  C12Q2565/501

摘要： The present invention is directed to a method of designing a plurality of capture oligonucleotide probes for use on a support to which complementary oligonucleotide probes will hybridize with little mismatch, where the plural capture oligonucleotide probes have melting temperatures within a narrow range. The present invention further relates to an oligonucleotide array comprising of a support with the plurality of oligonucleotide probes immobilized on the support, a method of using the support to detect single-base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences, and a kit for such detection, which includes the support on which the oligonucleotides have been immobilized.

公开(公告)号：US20180002735A1

公开(公告)日：2018-01-04

申请号：US15457471

申请日：2017-03-13

申请人： Complete Genomics, Inc.

发明人： Radoje T. Drmanac ,  Matthew J. Callow

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6874 ,  C12Q2565/501 ,  C12Q2539/103 ,  C12Q2531/119

摘要： The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.

公开(公告)号：US20170306391A1

公开(公告)日：2017-10-26

申请号：US15511380

申请日：2015-09-17

申请人： VITO NV

发明人： Jef Hooyberghs ,  An Jacobs ,  Hanny Willems

IPC分类号： C12Q1/68 ,  G06F19/18 ,  G06F19/20

CPC分类号： C12Q1/6827 ,  C12Q1/6837 ,  C12Q2525/161 ,  C12Q2565/501 ,  G16B20/00 ,  G16B25/00 ,  C12Q2537/165 ,  C12Q2545/101

摘要： Provided herein are methods for determining the presence of a mutant of target polynucleotides in a sample solution by contacting the sample solution and a reference solution containing the target nucleotide with identical probe sets and then comparing the hybridization intensities of corresponding probes of the probe sets. The probes provide a varying complementarity to the target sequence so that a range of hybridization intensities for the hybridization between the target polynucleotide and the probes is covered.