公开(公告)号：US20240360494A1

公开(公告)日：2024-10-31

申请号：US18763579

申请日：2024-07-03

Applicant: 10x Genomics, Inc.

Inventor： Justin Costa ,  Felice Alessio Bava

IPC: C12Q1/6837 ,  C12Q1/6876

CPC classification number: C12Q1/6837 ,  C12Q1/6876

Abstract: Provided herein are methods, compositions, and kits for capturing a ligation product, in which the method can include covalently reacting a capture moiety of a capture probe with a reactive moiety of the ligation product to form a captured ligation product.

公开(公告)号：US20240302358A1

公开(公告)日：2024-09-12

申请号：US18431041

申请日：2024-02-02

Applicant: McMaster University

Inventor： Warren G Foster

IPC: G01N33/53 ,  A61K45/06 ,  C12Q1/6837

CPC classification number: G01N33/5308 ,  A61K45/06 ,  C12Q1/6837 ,  B01J2219/00529 ,  G01N2496/00 ,  G01N2800/364

Abstract: A method of detecting the expression level of miRNA markers in a biological sample obtained from a mammal is provided. The method incudes the steps of i) detecting the expression level of one or more miRNA markers selected from the group of miR-199a-3p, miR-143-3p, miR-340-5p, let-7b-5p, miR-21-5p, miR-17-5p, miR-20a-5p and miR-103a-3p, in the biological sample; ii) detecting the expression level of at least one miRNA reference marker selected from miR-148b-3p and miR-30e-5p in the biological sample; and iii) normalizing the expression level of the miRNA marker(s) against the expression level of the miRNA reference marker in the sample and in a control. The method is useful for the diagnosis of endometriosis, monitoring of patient response to treatment, and assessment of disease progression and/or severity.

公开(公告)号：US12084712B2

公开(公告)日：2024-09-10

申请号：US16848241

申请日：2020-04-14

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum ,  Jody Martin ,  Nidhanjali Bansal ,  James Ghadiali ,  Katherine Lazaruk ,  Gretchen Yinbon Lam

IPC: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6834 ,  C12Q1/6837

CPC classification number: C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  B82Y5/00 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2563/149 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2600/16 ,  G01N2458/10 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2563/131 ,  C12Q2563/179 ,  C12Q1/6869 ,  C12Q2563/131 ,  C12Q2563/179

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US12060604B2

公开(公告)日：2024-08-13

申请号：US18302421

申请日：2023-04-18

Applicant: 10x Genomics, Inc.

Inventor： Layla Katiraee ,  Michael Schnall-Levin ,  Christina Galonska ,  Marlon Stoeckius

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/154

Abstract: Provided herein are methods of identifying a methylation status of an analyte in a biological sample. Also provided herein are methods that combine identifying the methylation status with spatial technology to identify the location of a methylation status in a biological sample.

公开(公告)号：US12060554B2

公开(公告)日：2024-08-13

申请号：US17470284

申请日：2021-09-09

Applicant: Illumina, Inc.

Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874

CPC classification number: C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

公开(公告)号：US20240209425A1

公开(公告)日：2024-06-27

申请号：US18420208

申请日：2024-01-23

Applicant: 10x Genomics, Inc.

Inventor： Yifeng Yin ,  Michael Schnall-Levin ,  Eswar Prasad Ramachandran Iyer

IPC: C12Q1/6837

CPC classification number: C12Q1/6837

Abstract: The present disclosure relates to compositions and methods for generating capture probes on a substrate for identifying the location of analytes in a biological sample.

公开(公告)号：US11993814B2

公开(公告)日：2024-05-28

申请号：US18190884

申请日：2023-03-27

Applicant: Becton, Dickinson and Company

Inventor： Stephen P. A. Fodor ,  Glenn K. Fu

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B25/20 ,  G16B30/00 ,  G16B25/10

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B25/20 ,  G16B30/00 ,  G16B25/10 ,  C12Q1/6809 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US20240158838A1

公开(公告)日：2024-05-16

申请号：US18418967

申请日：2024-01-22

Applicant: 10x Genomics, Inc.

Inventor： Luigi Jhon Alvarado Martinez ,  Eswar Prasad Ramachandran Iyer

IPC: C12Q1/6837 ,  C12Q1/6816 ,  C12Q1/6827

CPC classification number: C12Q1/6837 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q2543/101 ,  C12Q2565/514

Abstract: Provided herein are methods of determining a location of a target nucleic acid in a biological sample.

公开(公告)号：US20240150852A1

公开(公告)日：2024-05-09

申请号：US18548190

申请日：2022-02-28

Applicant: International Iberian Nanotechnology Laboratory (INL) ,  Universidade do Minho

Inventor： Bruno Miguel BARROSO RODRIGUES DE ALMEIDA ,  Joana Rafaela LARA GUERREIRO ,  Neide Marina VIEIRA PEREIRA ,  João Pedro DOS SANTOS HALL AGORRETA ALPUIM ,  Jérôme Gilles OLLIVIER BORME ,  Pedro Eduardo MENDES FERREIRA ,  Rui César DE ALMEIDA CAMPOS

IPC: C12Q1/6893 ,  B01L3/00 ,  C12Q1/6837

CPC classification number: C12Q1/6893 ,  B01L3/502761 ,  C12Q1/6837 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0645 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/166

Abstract: The present disclosure relates to a monolayer graphene-based multiplex malaria diagnostic sensor. Specifically, a monolayer graphene-based sensor that is able to simultaneously detect the presence of different Plasmodium species, presence of drug-resistant Plasmodium species, and also the presence of a relevant polymorphism in a subject. The present disclosure also relates to a monolayer graphene-based sensor, method and kit for a rapid diagnosis of malaria using a non-invasive biological sample obtained from a subject, preferably in saliva or urine samples.

公开(公告)号：US11970785B2

公开(公告)日：2024-04-30

申请号：US17460513

申请日：2021-08-30

Applicant: Base Pair Biotechnologies, Inc.

Inventor： George W. Jackson ,  Robert Batchelor ,  Alexander S. Chiu ,  Rafal Drabek ,  Deepak Thirunavukarasu ,  Caitlin Bruns

IPC: C07H21/04 ,  C12Q1/6811 ,  C12Q1/6837 ,  C40B40/06

CPC classification number: C40B40/06 ,  C12Q1/6811 ,  C12Q1/6837 ,  C12Q1/6811 ,  C12Q2525/205 ,  C12Q2525/301 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/101 ,  C12Q2565/501 ,  C12Q1/6837 ,  C12Q2525/205 ,  C12Q2525/301 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/101

Abstract: This invention relates to methods and materials for multiplexed utilization of collections of functional ligands, particularly to methods and materials for selecting for and/or utilizing particular desirable traits of functional ligands in a multiplexed manner, and more particularly to methods and materials for selecting for and/or utilizing particular structural changes of functional ligands in a multiplexed manner.