公开(公告)号：US10087492B2

公开(公告)日：2018-10-02

申请号：US15705447

申请日：2017-09-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  W. Michael Ainley ,  Jayakumar P. Samuel ,  Zehui Cao ,  Liu Shen ,  Cristie M. Dewes

IPC: C12P19/34 ,  C12Q1/6895

Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.