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公开(公告)号：US10344322B2
公开(公告)日：2019-07-09
申请号：US15249684
申请日：2016-08-29
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew A. Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
IPC: C12Q1/68 , C12Q1/6858 , C12Q1/6895 , C12N15/10 , C12N15/66 , C12N15/70 , C40B40/08 , A01G24/00 , C12N5/00
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
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公开(公告)号：US20180371478A1
公开(公告)日：2018-12-27
申请号：US16123326
申请日：2018-09-06
Applicant: DOW AGROSCIENCES LLC
Inventor： Lakshmi Sastry-Dent , Zehui Cao , Shreedharan Sriram , Steven R. Webb , Debra L. Camper , W. Michael Ainley
Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.
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3.发明申请AUTOMATION OF BARRIER-BASED PLANT NUCLEIC ACID AND PROTEIN EXTRACTION 审中-公开基于BARRIER的植物核酸和蛋白质提取的自动化公开(公告)号：US20150299766A1
公开(公告)日：2015-10-22
申请号：US14380584
申请日：2013-02-22
Applicant: Dow AgroSciences LLC
Inventor： Alice Wernicki , Lakshmi Sastry-Dent , Delkin Orlando Gonzalez
CPC classification number: C12Q1/6806 , B01L3/502753 , B01L2200/0668 , B01L2300/0803 , B01L2400/0409 , B01L2400/043 , C12M47/06 , C12N15/1013 , G01N1/4005 , G01N1/4077 , G01N35/0098 , G01N35/0099 , G01N2001/4016
Abstract: This invention is related to systems and methods for nucleic acid/protein extraction/isolation from biological samples. In particular, automated barrier-based systems for samples from plant tissues/cells are disclosed. The automated systems and methods disclosed provide reliability and high throughput specifically suitable for nucleic acid/protein extraction/isolation from plant tissues/cells. In some embodiments, the automated systems disclosed can further enhance high throughput capacity for nucleic acid/protein extraction/isolation directly from plant tissue samples.
Abstract translation: 本发明涉及从生物样品中进行核酸/蛋白质提取/分离的系统和方法。 特别地，公开了用于来自植物组织/细胞的样品的基于自动化的基于屏障的系统。 所公开的自动化系统和方法提供了可靠性和高通量，其特别适用于从植物组织/细胞中提取/分离核酸/蛋白质。 在一些实施方案中，所公开的自动化系统可以进一步增强来自植物组织样品的核酸/蛋白质提取/分离的高通量能力。
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公开(公告)号：US20150064708A1
公开(公告)日：2015-03-05
申请号：US14475968
申请日：2014-09-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , W. Michael AINLEY , Jayakumar P. SAMUEL , Zehui CAO , Liu Y. SHEN , Cristie M. DEWES
IPC: C12Q1/68
CPC classification number: C12Q1/6895
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座上的供体DNA多核苷酸插入物的高通量过程。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。
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公开(公告)号：US20130130920A1
公开(公告)日：2013-05-23
申请号：US13683442
申请日：2012-11-21
Applicant: Dow AgroSciences LLC
Inventor： Zehui Cao , Stephen Novak , Lakshmi Sastry-Dent , Ning Zhou
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6895
Abstract: A method of analyzing, in chromosomal DNA having a transgene incorporated therein, a DNA flanking region derived from the chromosome which is adjacent to the transgene. Wherein, the DNA flanking region is characterized by isolation and digestion of genomic DNA with a restriction enzyme, ligation of a double stranded adapter to the isolated and digested genomic DNA, a primer extension reaction of the adapter ligated genomic DNA, and the isolation of the primer extension reaction product via a streptavidin-biotin interaction. The DNA flanking region is further characterized via subsequent PCR amplification reactions and DNA sequencing.
Abstract translation: 在其中掺入转基因的染色体DNA中分析衍生自与转基因相邻的染色体的DNA侧翼区的方法。 其中DNA侧翼区的特征在于用限制酶分离和消化基因组DNA，将双链衔接子连接到分离和消化的基因组DNA上，衔接子连接的基因组DNA的引物延伸反应和 通过链霉亲和素 - 生物素相互作用引物延伸反应产物。 通过随后的PCR扩增反应和DNA测序进一步表征DNA侧翼区。
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Patent Agency Ranking
公开(公告)号：US10273493B2
公开(公告)日：2019-04-30
申请号：US14531705
申请日：2014-11-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Zehui Cao , Shreedharan Sriram , Steven R. Webb , Debra L. Camper , Navin Elango
Abstract: As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.
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公开(公告)号：US20170130266A1
公开(公告)日：2017-05-11
申请号：US15333574
申请日：2016-10-25
Applicant: Dow AgroSciences LLC
Inventor： Pohao Wang , Shreedharan Sriram , Lakshmi Sastry-Dent
CPC classification number: C12Q1/6876 , C12N15/82 , C12Q1/6895 , C12Q2600/154 , C12Q2600/158 , G16B20/00 , G16B25/00
Abstract: The present invention is based on the use of the H3K9me2 methylation levels in plant genomes to predict transgene silencing, transgene stability, and/or transgene expression level. Provided are methods and/or systems for generating whole-genome H3K9me2 maps and its use with an assigned threshold value for predicting gene silencing. The methods and/or systems provided herein can be used in high-throughput setting for screening large number of transformed event in a relatively short period of time as compared to existing technologies.
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公开(公告)号：US09523098B2
公开(公告)日：2016-12-20
申请号：US13875992
申请日：2013-05-02
Applicant: Dow AgroSciences LLC , Sangamo BioSciences, Inc.
Inventor： Vipula Shukla , Manju Gupta , Fyodor Urnov , Dmitry Guschin , Michiel Jan De Both , Paul Bundock , Lakshmi Sastry-Dent
CPC classification number: C12N9/0006 , A01C14/00 , C12N15/8213 , C12N15/8241 , C12N15/8243 , C12N15/8261 , C12N15/8269 , C12Y101/01037 , C12Y301/00 , Y02A40/146
Abstract: Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.
Abstract translation: 本文公开了用于靶向修饰一种或多种内源性植物苹果酸脱氢酶基因的方法和组合物。
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公开(公告)号：US09493844B2
公开(公告)日：2016-11-15
申请号：US14105223
申请日：2013-12-13
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
IPC: C12Q1/68
CPC classification number: C12Q1/6858 , A01G24/00 , C12N5/00 , C12N15/1082 , C12N15/66 , C12N15/70 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B40/08
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。
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10.发明申请METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION OF TRANSGENES 审中-公开用于核转移介导的靶向转移整合的方法和组合物公开(公告)号：US20130326645A1
公开(公告)日：2013-12-05
申请号：US13889162
申请日：2013-05-07
Applicant: Dow AgroSciences LLC , Sangamo BioSciences, Inc.
Inventor： Gregory J. Cost , Fyodor Urnov , William M. Ainley , Joseph F. Petolino , Jayakumar Pon Samuel , Steven R. Webb , Lakshmi Sastry-Dent
CPC classification number: C12N15/8201 , C12N15/102 , C12N15/8209 , C12N15/8213 , C12N15/8216 , C12N15/8241 , C12N15/8247 , C12N15/8274 , C12N15/8278 , C12N15/8286 , C12N15/85 , C12N15/87 , C12N2800/80 , Y02A40/162
Abstract: Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.
Abstract translation: 本文公开了用于与供体分子同源性独立的靶向插入细胞基因组的方法和组合物。
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