公开(公告)号：US20190316141A1

公开(公告)日：2019-10-17

申请号：US16392217

申请日：2019-04-23

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： W. Michael Ainley ,  Steven R. Webb ,  Jayakumar P. Samuel ,  Dmitry Y. Guschin ,  Jeffrey C. Miller ,  Lei Zhang

IPC: C12N15/82 ,  C07K14/47 ,  C12N15/10 ,  C12N9/22 ,  C12N15/90 ,  C12N15/66

Abstract: Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

公开(公告)号：US20140173774A1

公开(公告)日：2014-06-19

申请号：US14134883

申请日：2013-12-19

Applicant: Dow AgroSciences LLC

Inventor： Dayakar Pareddy ,  Sivarama R. Chennareddy ,  Tatyana Minnicks ,  Olga Karpova ,  David Griffin ,  Jayakumar P. Samuel ,  Kelley Smith ,  Rodrigo Sarria-Milan

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8286 ,  A01H5/00 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8283

Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.

Abstract translation: 公开了一种用于土壤杆菌介导的大豆种系转化的方法，包括用含有转基因的根癌土壤杆菌感染具有一部分胚轴的分裂大豆种子。 所述方法还可以包括通过在选择培养基上体外包含部分胚胎轴的裂解大豆种子转化产生的外植体再生。

公开(公告)号：US20170044559A1

公开(公告)日：2017-02-16

申请号：US15292774

申请日：2016-10-13

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： W. Michael Ainley ,  Steven R. Webb ,  Jayakumar P. Samuel ,  Dmitry Y. Guschin ,  Jeffrey C. Miller ,  Lei Zhang

IPC: C12N15/82 ,  C07K14/47 ,  C12N9/22

CPC classification number: C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

Abstract: Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

Abstract translation: FAD2基因座中的基因破坏，基因编辑或基因堆叠的方法和组合，通过以位点方式切割大豆细胞中FAD2基因的位置，在FAD2基因中产生断裂，然后任选地整合入 揭示目的核酸分子。

公开(公告)号：US08916752B2

公开(公告)日：2014-12-23

申请号：US13647081

申请日：2012-10-08

Applicant: Dow AgroSciences LLC

Inventor： Terry R. Wright ,  Justin M. Lira ,  Terence Anthony Walsh ,  Donald Merlo ,  Jayakumar P. Samuel ,  Gaofeng Lin

IPC: C12N15/82

CPC classification number: C12N15/8274 ,  C12N9/0071 ,  C12N15/8275

Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

Abstract translation: 本发明提供了不仅对2,4-D有抗性的新型植物，而且还提供了对吡啶氧基乙酸酯除草剂的抗性。 迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。 本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。 本发明能够以新的方式使用除草剂的新型组合。 此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。 根据本发明使用的优选酶和基因在本文中称为AAD-12（丙烯酰氧基烷酸酯加氧酶）。 这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。

公开(公告)号：US10287595B2

公开(公告)日：2019-05-14

申请号：US15292774

申请日：2016-10-13

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： W. Michael Ainley ,  Steven R. Webb ,  Jayakumar P. Samuel ,  Dmitry Y. Guschin ,  Jeffrey C. Miller ,  Lei Zhang

IPC: C12N15/82 ,  C07K14/00 ,  C12N15/66 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90 ,  C07K14/47

Abstract: Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

公开(公告)号：US09765404B2

公开(公告)日：2017-09-19

申请号：US14475968

申请日：2014-09-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  W. Michael Ainley ,  Jayakumar P. Samuel ,  Zehui Cao ,  Liu Y. Shen ,  Cristie M. Dewes

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6895

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain donor sequences inserted precisely into targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce a first amplicon from donor sequences inserted in the reverse orientation, amplifying the first amplicon with a second round of PCR and detecting the presence of the second amplicon, wherein the production of the first and second amplicon indicates the presence of the site specific integration event.

公开(公告)号：US10087492B2

公开(公告)日：2018-10-02

申请号：US15705447

申请日：2017-09-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi Sastry-Dent ,  W. Michael Ainley ,  Jayakumar P. Samuel ,  Zehui Cao ,  Liu Shen ,  Cristie M. Dewes

IPC: C12P19/34 ,  C12Q1/6895

Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

公开(公告)号：US20140173780A1

公开(公告)日：2014-06-19

申请号：US14133370

申请日：2013-12-18

Applicant: DOW AGROSCIENCES LLC

Inventor： Dayakar Pareddy ,  Sivarama R. Chennareddy ,  Tatyana Minnicks ,  Olga Karpova ,  David Griffin ,  Jayakumar P. Samuel ,  Kelley A. Smith ,  Rodrigo Sarria-Millan ,  Tejinder Kumar Mall

IPC: C12N15/82

CPC classification number: C12N15/8286 ,  A01H5/00 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8283

Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.