公开(公告)号：US06645741B2

公开(公告)日：2003-11-11

申请号：US10015593

申请日：2001-12-17

申请人： Detlef Kozian ,  Birgit Reuner

发明人： Detlef Kozian ,  Birgit Reuner

IPC分类号： C12P1934

CPC分类号： C12Q1/6853 ,  C12Q1/6809 ,  Y10T436/143333 ,  C12Q2565/102 ,  C12Q2525/173 ,  C12Q2521/107

摘要： The invention relates to a novel method for analyzing the composition of an mRNA sample and analyzing differential gene expression using two differently labeled primers, and to the use of the method. In the method for analyzing an RNA sample, a) a first primer, which is, where appropriate, labeled with a first dye, is used to prepare the first strand of a complementary DNA sample or cDNA sample from an RNA sample, b) a second primer, which is preferably labeled with a second dye, is used to prepare the second strand of this cDNA sample, c) the first primer, which is labeled with a first dye, and the second primer, which is labeled with a second dye, are used to amplify the cDNA sample, and d) the composition of the amplified, labeled cDNA sample is analyzed.

摘要翻译： 本发明涉及一种用于分析mRNA样品的组成并使用两个不同标记的引物分析差异基因表达的新方法以及该方法的使用。在分析RNA样品的方法中，a）第一引物，其中 在适当的情况下用第一染料标记，用于从RNA样品制备互补DNA样品或cDNA样品的第一条链，b）优选用第二染料标记的第二引物用于制备 该cDNA样品的第二条链，c）用第一染料标记的第一引物，用第二染料标记的第二引物用于扩增cDNA样品，并且d）扩增的 分析标记的cDNA样品。

公开(公告)号：US06342376B1

公开(公告)日：2002-01-29

申请号：US09390324

申请日：1999-09-07

申请人： Detlef Kozian ,  Birgit Reuner

发明人： Detlef Kozian ,  Birgit Reuner

IPC分类号： C12D1934

CPC分类号： C12Q1/6853 ,  C12Q1/6809 ,  Y10T436/143333 ,  C12Q2565/102 ,  C12Q2525/173 ,  C12Q2521/107

摘要： The invention relates to a novel method for analyzing the composition of an mRNA sample and analyzing differential gene expression using two differently labeled primers, and to the use of the method. In the method for analyzing an RNA sample, a) a first primer, which is, where appropriate, labeled with a first dye, is used to prepare the first strand of a complementary DNA sample or cDNA sample from an RNA sample, b) a second primer, which is preferably labeled with a second dye, is used to prepare the second strand of this cDNA sample, c) the first primer, which is labeled with a first dye, and the second primer, which is labeled with a second dye, are used to amplify the cDNA sample, and d) the composition of the amplified, labeled cDNA sample is analyzed.

摘要翻译： 本发明涉及一种用于分析mRNA样品的组成并使用两个不同标记的引物分析差异基因表达的新方法，以及该方法的使用。 在分析RNA样品的方法中，a）在适当时用第一染料标记的第一引物用于从RNA样品制备互补DNA样品或cDNA样品的第一条链，b） 优选用第二染料标记的第二引物用于制备该cDNA样品的第二链，c）用第一染料标记的第一引物和用第二染料标记的第二引物 ，用于扩增cDNA样品，并且d）分析扩增的标记的cDNA样品的组成。