公开(公告)号：US20150184215A1

公开(公告)日：2015-07-02

申请号：US14584836

申请日：2014-12-29

Applicant: Development Center for Biotechnology

Inventor： Yu-Shen Hsu ,  Show-Shan Sheu ,  Bo-Chin Lei ,  Tsan-Hui Wu

IPC: C12P21/00 ,  C07K1/20 ,  C07K14/34 ,  C07K1/18 ,  C07K1/22

CPC classification number: C07K1/20 ,  C07K14/34 ,  C12P21/02

Abstract: A method for recombinant production of a CRM197 protein includes culturing a recombinant Escherichia coli cell to produce said CRM197 protein, and isolating said CRM197 protein. The recombinant Escherichia coli cell includes an expression vector, which contains a nucleic acid molecule that encodes a fusion protein that includes an E. coli periplasmic signal peptide at N terminal and the CRM197 at C terminal. The CRM197 is encoded by a polynucleotide having the sequence of SEQ ID NO: 1. The E. coli periplasmic signal peptide comprise a pelB leader sequence. The nucleic acid molecule comprises the sequence of SEQ ID NO:2. The Escherichia coli cell is BL21(DE3)pLysS.

Abstract translation: 重组生产CRM197蛋白的方法包括培养重组大肠杆菌细胞以产生所述CRM197蛋白，并分离所述CRM197蛋白。 重组大肠杆菌细胞包含表达载体，其含有编码融合蛋白的核酸分子，所述融合蛋白包括N末端的大肠杆菌周质信号肽和C末端的CRM197。 CRM197由具有SEQ ID NO：1的序列的多核苷酸编码。大肠杆菌周质信号肽包含pelB前导序列。 核酸分子包含SEQ ID NO：2的序列。 大肠杆菌细胞是BL21（DE3）pLysS。