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公开(公告)号:US20190233499A1
公开(公告)日:2019-08-01
申请号:US16335519
申请日:2017-09-21
发明人: Kouta HATAYAMA , Teruhiko IDE , Hiroyuki ITO , Yosuke TERAO , Naoki YAMANAKA , Satoshi ENDO
CPC分类号: C07K14/70535 , C07K1/22 , C07K16/065 , C12N1/20 , C12N15/70 , C12P21/02
摘要: The problem to be addressed by the present invention is to provide improved recombinant FcγRIIb and FcγRIIa that do not require refolding and exhibit high productivity and thermal stability, and to provide a method for producing the same. Said problem is solved by improved recombinant FcγRIIb comprising at least the amino acid residues of the extracellular domain of human FcγRIIb (No. 43 to No. 215 in UniProt No. P31994), wherein, in said amino acid residues, at least one amino acid substitution has occurred at a position corresponding to No. 82, 94, 98, 104, 105, or 139 in UniProt No. P31994. Said problem is also solved by improved recombinant FcγRIIa comprising at least the amino acid residues of the extracellular domain of human FcγRIIa (No. 34 to No. 206 in UniProt No. P12318-1), wherein, in said amino acid residues, at least one amino acid substitution has occurred at a position corresponding to No. 73, 85, 89, 95, 96, or 130 in UniProt No. P12318-1.
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公开(公告)号:US20190218247A1
公开(公告)日:2019-07-18
申请号:US16103812
申请日:2018-08-14
申请人: Barofold, Inc.
发明人: Matthew Seefeldt , Eliana Gomez
IPC分类号: C07K1/14 , C12N9/50 , C07K14/555 , C12P21/02 , C07K14/54
CPC分类号: C07K1/14 , C07K1/1136 , C07K1/145 , C07K14/54 , C07K14/555 , C12N9/506 , C12P21/02
摘要: The invention provides methods and systems for production of recombinant protein, and particularly, for production of recombinant protein from inclusion bodies. For example, in one aspect, the method comprises providing a protein preparation comprising inclusion bodies, preparing an inclusion body dispersion, and exposing the protein preparation to high pressure in a pressure vessel, to disaggregate and refold the inclusion body protein.
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公开(公告)号:US20190212347A1
公开(公告)日:2019-07-11
申请号:US16070651
申请日:2017-01-20
申请人: BIOCON LIMITED
发明人: MADHAVAN BUDDHA , MUKESH B. PATALE , ANAND KHEDKAR , RANITENDRANATH TAGORE , SEBASTIAN ALASTAIR MCDONALD
CPC分类号: G01N33/74 , C12P21/02 , G01N33/6848 , G01N2333/62 , G01N2458/15
摘要: The present invention provides for a specific and sensitive bio-analytical method for detection of insulin or insulin analogues in plasma, serum or any other biological fluid, wherein the insulin or insulin analogues are labelled with a stable isotopic nitrogen for detection by the use of solid phase extraction and liquid chromatography with tandem mass spectrometric detection.
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公开(公告)号:US20190211315A1
公开(公告)日:2019-07-11
申请号:US16242904
申请日:2019-01-08
申请人: DNA Script
发明人: Elise Champion , Mikhael Soskine , Thomas Ybert , Marc Delarue
IPC分类号: C12N9/12
CPC分类号: C12N9/1264 , C12P19/34 , C12P21/02 , C12Y207/07031
摘要: The present invention relates to a variant of Terminal deoxynucleotidyl Transferase (TdT) which (i) comprises the amino acid sequence as set forth in SEQ ID No 2 or a functionally equivalent sequence, with at least an amino acid substitution at position corresponding to residue C302 or functionally equivalent residue, wherein the position is numbered by reference to the amino acid sequence set forth in SEQ ID No 1, (ii) is able to synthesize a nucleic acid fragment without template and (iii) is able to incorporate a modified nucleotide into the nucleic fragment.
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公开(公告)号:US20190211077A2
公开(公告)日:2019-07-11
申请号:US15321916
申请日:2015-06-24
申请人: Yoshiharu ASAOKA , Toru TANAKA , Yosuke TERAO , Naoki YAMANAKA , Natsuko KIZU , Masaru AOKI , Teruhiko IDE
发明人: Yoshiharu ASAOKA , Toru TANAKA , Yosuke TERAO , Naoki YAMANAKA , Natsuko KIZU , Masaru AOKI , Teruhiko IDE
IPC分类号: C07K14/735 , C07K1/22 , C07K16/00 , B01J20/281 , G01N33/68 , B01D15/38
CPC分类号: C07K14/70535 , B01D15/3809 , C07K1/22 , C07K16/00 , C07K16/065 , C07K2317/10 , C07K2317/41 , C07K2317/732 , C12N15/09 , C12P21/02 , G01N30/482 , G01N33/6854 , G01N2030/486 , G01N2400/02
摘要: Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.
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公开(公告)号:US20190203249A1
公开(公告)日:2019-07-04
申请号:US16325583
申请日:2017-08-15
CPC分类号: C12P21/02 , C12N5/0682 , C12N9/0069 , C12N15/52 , C12Y113/11027
摘要: The present invention relates to modified producer cells for improved production of therapeutic proteins. Specifically, the inventors have found that removing genes involved in amino acid catabolism in Chinese Hamster Ovary (CHO) cells improves the cell growth and viability and likely also the yield of a recombinant therapeutic protein produced by the cells.
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7.
公开(公告)号:US20190201496A1
公开(公告)日:2019-07-04
申请号:US16333918
申请日:2017-09-14
发明人: Richard S. BLUMBERG , Michal PYZIK , Amit GANDHI , Inger SANDLIE , Kine Marita Knudsen SAND , Jan Terje ANDERSEN
IPC分类号: A61K38/38 , C07K14/47 , C12P21/02 , A61P37/06 , C07K14/765
CPC分类号: A61K38/38 , A61P37/06 , C07K14/47 , C07K14/4715 , C07K14/70539 , C07K14/765 , C07K16/2833 , C07K19/00 , C12P21/02
摘要: Provided herein, in some aspects, are compositions and methods to inhibit AFP interactions with β2M and/or Class I-related molecule interactions in diseases or disorders where elevated AFP levels are associated with immunosuppression. Also provided herein, in some aspects, are compositions and methods to enhance or potentiate AFP interactions with β2M and/or Class I-related molecule in diseases or disorders with decreased AFP levels or diseases or disorders where increasing AFP levels is desired to increase immunosuppression or enhance organ regeneration.
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公开(公告)号:US20190194647A1
公开(公告)日:2019-06-27
申请号:US16313613
申请日:2017-06-29
申请人: Zymergen Inc.
发明人: Shawn Manchester , Jeffrey Mellin
CPC分类号: C12N15/1037 , C07K1/047 , C12N9/1205 , C12P1/04 , C12P19/02 , C12P21/02 , C12Y207/01001 , C12Y207/01069
摘要: The present disclosure describes methods for generating microbial strains expressing a heterologous bacterial glucose permease gene that produce biomolecules of interest. In aspects, the disclosure provides novel bacterial strains, which express a heterologous bacterial glucose permease gene whose expression is controlled by a native Corynebacterium glutamicum promoter or a mutant promoter derived therefrom. Also provided herein are methods for producing a library of bacterial glucose permease genes using a promoter ladder comprising a plurality of promoters derived from Corynebacterium glutamicum.
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公开(公告)号:US20190119690A1
公开(公告)日:2019-04-25
申请号:US16094795
申请日:2017-04-18
申请人: Novozymes A/S
发明人: Hiroshi Teramoto
CPC分类号: C12N15/80 , C07K14/37 , C07K14/38 , C12N9/2428 , C12P21/02
摘要: The present invention relates to RImA-inactivated filamentous fungal cells secreting a polypeptide of interest and methods of producing a secreted polypeptide of interest in said cells as well as methods of producing said cells.
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10.
公开(公告)号:US20180346901A1
公开(公告)日:2018-12-06
申请号:US15780187
申请日:2016-11-29
IPC分类号: C12N9/00 , C12N15/67 , C12N15/52 , C12N15/86 , C12N5/07 , C07K16/18 , C07K16/32 , C07K14/47 , C07K16/24
CPC分类号: C12N15/09 , C12N9/00 , C12N15/52 , C12N15/63 , C12N15/67 , C12N15/86 , C12N2710/14143 , C12P13/005 , C12P21/02
摘要: The invention relates to a method of preparing engineered target polypeptides (TP) comprising in its amino acid sequence one or more, identical or different, non-canonical amino acid (ncAA) residues, by expressing said TP in an insect cell line (ICL) and by expressing novel orthogonal bacterial aminoacyl tRNA synthetase/tRNA pairs in said ICL; a baculoviral shuttle vector (bacmid) suitable or introducing the genetic information of said orthogonal tRNA synthetase/tRNA into said ILC; particular expression cassettes for expressing said particular tRNAs in said ILC; TPs obtained by said method; as we as a kit for preparing said TPs.
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