公开(公告)号：US09677080B2

公开(公告)日：2017-06-13

申请号：US14413930

申请日：2012-11-15

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Xiangshan Zhou ,  Yuanxing Zhang ,  Jinjia Wang ,  Xiaolong Wang ,  Peng Bai ,  Ping Zhang

IPC: C12P21/06 ,  C12N1/00 ,  C12N15/81 ,  C07K14/39 ,  C12N1/32 ,  C12P21/00

CPC classification number: C12N15/815 ,  C07K14/39 ,  C12N1/32 ,  C12P21/00

Abstract: Provided is a method of eliminating the dependence of methanol induced promoter on a single methanol carbon source for expressing foreign polypeptide. The method comprises activating the expression of the promoter requiring methanol induction by increasing the expression quantity of Mit1 polypeptide in cells of methylotrophic yeast, so that the promoter originally depending on methanol induction no longer depends on single methanol and can also express foreign polypeptide.

公开(公告)号：US20150299716A1

公开(公告)日：2015-10-22

申请号：US14413930

申请日：2012-11-15

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Xiangshan Zhou ,  Yuanxing Zhang ,  Jinjia Wang ,  Xiaolong Wang ,  Peng Bai ,  Ping Zhang

IPC: C12N15/81 ,  C12P21/00

CPC classification number: C12N15/815 ,  C07K14/39 ,  C12N1/32 ,  C12P21/00

Abstract: Provided is a method of eliminating the dependence of methanol induced promoter on a single methanol carbon source for expressing foreign polypeptide. The method comprises activating the expression of the promoter requiring methanol induction by increasing the expression quantity of Mit1 polypeptide in cells of methylotrophic yeast, so that the promoter originally depending on methanol induction no longer depends on single methanol and can also express foreign polypeptide.

Abstract translation: 提供了消除甲醇诱导的启动子对用于表达外来多肽的单一甲醇碳源的依赖性的方法。 该方法包括通过增加甲基营养酵母细胞中Mit1多肽的表达量来激活需要甲醇诱导的启动子的表达，使得最初依赖于甲醇诱导的启动子不再取决于单一甲醇，并且还可以表达外源多肽。