公开(公告)号：US20190241584A1

公开(公告)日：2019-08-08

申请号：US16318762

申请日：2017-07-18

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Linyong Zhu ,  Yi Yang ,  Dasheng Zhang ,  Zengmin Du ,  Bingkun Bao ,  Qiuning Lin ,  Yan Li ,  Xianjun Chen ,  Lipeng Yang ,  Chunyan Bao

IPC: C07D495/04 ,  C07D333/78 ,  C07D495/14 ,  C09B57/00 ,  G01N33/68 ,  C12Q1/68 ,  G01N33/533 ,  C09B23/04 ,  C09B23/01

CPC classification number: C07D495/04 ,  A61K49/00 ,  C07D333/60 ,  C07D333/78 ,  C07D495/14 ,  C09B23/0058 ,  C09B23/0091 ,  C09B23/04 ,  C09B57/00 ,  C09K11/06 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/68

Abstract: Provided are a fluorescent dye and a preparation process and use thereof. The fluorescent dye is able to emit a long wavelength, is sensitive and specific to viscosity, and is used for a wide range of purposes, such as viscosity testing, fluorescent labeling, quantification or detection of proteins, enzymes or nucleic acids. In addition, it can be used as a fluorescent-activated probe.

公开(公告)号：US20190187144A1

公开(公告)日：2019-06-20

申请号：US16318868

申请日：2017-07-18

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Linyong Zhu ,  Yi Yang ,  Dasheng Zhang ,  Zengmin Du ,  Bingkun Bao ,  Qiuning Lin ,  Xianjun Chen ,  Lipeng Yang ,  Chunyan Bao ,  Yihui Ge ,  Renmei Liu ,  Zhengda Chen ,  Sitong Zhang ,  Ningfeng Li ,  Xin Hua

IPC: G01N33/58 ,  G01N33/533 ,  C09K11/06 ,  A61K49/00 ,  G01N21/64 ,  C07D487/04 ,  C07D263/56 ,  C07D277/64 ,  C07D495/04 ,  C07F7/08 ,  C07D409/14

CPC classification number: G01N33/582 ,  A61K49/00 ,  A61K49/0017 ,  C07D263/56 ,  C07D277/64 ,  C07D409/14 ,  C07D473/18 ,  C07D487/04 ,  C07D495/04 ,  C07F7/0816 ,  C09K11/06 ,  G01N21/6428 ,  G01N33/533

Abstract: Provided are a fluorescent probe and a preparation process and the use thereof. The fluorescent probe is sensitive and specific to viscosity, and can be used for specific fluorescent labeling of proteins, and can also be used for quantification, detection or kinetic studies of proteins, and the imaging of cells, tissues and living bodies.

公开(公告)号：US10184126B2

公开(公告)日：2019-01-22

申请号：US14419106

申请日：2013-08-01

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Yi Yang ,  Xianjun Chen ,  Zhengcai Ma ,  Renmei Liu

IPC: C12N15/62 ,  C12N15/70 ,  C12N15/74 ,  C12N15/63 ,  C12N15/75 ,  C12P21/02 ,  C12N9/02 ,  C12N13/00

Abstract: Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system. The optically controlled gene expression system of prokaryotic bacterium has a quick, effective and powerful induction, is safer than other inducers, is of little or no toxicity, and can control gene expression both spatially and temporally, and can regulate many life processes of prokaryotic bacterium.