公开(公告)号：US20160040173A1

公开(公告)日：2016-02-11

申请号：US14419106

申请日：2013-08-01

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： YI YANG ,  XIANJUN CHEN ,  ZHENGCAI MA ,  RENMEI LIU

IPC: C12N15/63 ,  C12N15/70 ,  C12N13/00

CPC classification number: C12N15/635 ,  C12N9/0051 ,  C12N13/00 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75 ,  C12P21/02

Abstract: Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system. The optically controlled gene expression system of prokaryotic bacterium has a quick, effective and powerful induction, is safer than other inducers, is of little or no toxicity, and can control gene expression both spatially and temporally, and can regulate many life processes of prokaryotic bacterium.

Abstract translation: 提供了一种原核细菌的光学控制基因表达系统，其包含：a）感光重组转录因子编码基因，所述感光重组转录因子是包含第一多肽作为DNA结合结构域的第一种融合蛋白，第二多肽作为感光结构域 ; b）包含启动子或启动子 - 反应元件或含有至少一个被第一多肽识别/结合的反应元件和待转录的核酸序列的反应元件的靶转录单元。 还提供了包含所述光学控制的基因表达系统的原核表达载体和通过使用光学控制的基因表达系统调节原核宿主细胞中的基因表达的方法。 还提供了含有光学控制的基因表达系统的不同成分的试剂盒。 原核细菌的光学控制基因表达系统具有快速，有效和强大的诱导，比其他诱导物更安全，毒性很小或无毒，可以在空间和时间上控制基因表达，并且可以调节原核细菌的许多生命过程 。