公开(公告)号：US08541224B2

公开(公告)日：2013-09-24

申请号：US13732870

申请日：2013-01-02

Applicant: EMD Millipore Corporation

Inventor： Shawn Bates ,  Kerry Roche Lentine ,  Nada Bsat

IPC: C12N1/20 ,  C12Q1/02 ,  C12Q1/22

CPC classification number: C12N1/20 ,  C12Q1/02 ,  C12Q1/22

Abstract: A method for retention testing sterilizing grade filters comprises: a) providing a stock of Acholeplasma laidlawii; b) growing up the stock of A. laidlawii for about 24 hours or less in a single serum-free growth medium that supports cell growth to a high titer and yields a cellular morphology where the cells are small, deaggregated and spherical, thereby producing a bacterial culture; c) challenging a test filter by filtering the bacterial culture through the test filter at a known challenge level, thereby producing a filtrate downstream of the test filter; and d) detecting concentration of A. laidlawii in the filtrate. Serum-free growth media for cultivating or storing A. laidlawii are also described.

公开(公告)号：US20130130356A1

公开(公告)日：2013-05-23

申请号：US13732870

申请日：2013-01-02

Applicant: EMD MILLIPORE CORPORATION

Inventor： Shawn Bates ,  Kerry Roche Lentine ,  Nada Bsat

IPC: C12N1/20

CPC classification number: C12N1/20 ,  C12Q1/02 ,  C12Q1/22

Abstract: A method for retention testing sterilizing grade filters comprises: a) providing a stock of Acholeplasma laidlawii; b) growing up the stock of A. laidlawii for about 24 hours or less in a single serum-free growth medium that supports cell growth to a high titer and yields a cellular morphology where the cells are small, deaggregated and spherical, thereby producing a bacterial culture; c) challenging a test filter by filtering the bacterial culture through the test filter at a known challenge level, thereby producing a filtrate downstream of the test filter; and d) detecting concentration of A. laidlawii in the filtrate. Serum-free growth media for cultivating or storing A. laidlawii are also described.

Abstract translation: 一种杀菌级过滤器的保留测试方法包括：a）提供普通Ac a草; b）在支持细胞生长至高滴度的单一无血清生长培养基中培养约24小时或更少的A.laylawii的存量，并产生细胞形态，其中细胞小，解聚和球形，从而产生 细菌培养; c）通过在已知的攻击级别通过测试过滤器过滤细菌培养物来挑战测试过滤器，从而在测试过滤器的下游产生滤液; 和d）检测滤液中的嗜。菌浓度。 还描述了用于培养或储存A.lllawii的无血清生长培养基。