公开(公告)号：US09243286B2

公开(公告)日：2016-01-26

申请号：US14153261

申请日：2014-01-13

Applicant: Gen-Probe Incorporated

Inventor： Lyle J. Arnold, Jr. ,  Bob D. Brown

IPC: C12Q1/68

CPC classification number: C12Q1/6834 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2525/197 ,  C12Q2525/161

Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.

Abstract translation: 本发明涉及包含分子开关的寡核苷酸，分子开关可以存在于“开放”或“闭合”位置。 探针的分子开关部分对与分子开关互补的序列的身份特别敏感。 含有分子开关的寡核苷酸适用于各种杂交过程。 由于寡核苷酸的开关结构域的灵敏度，含有分子开关的探针在识别单点错配中特别有用。 更具体地，寡核苷酸的一部分但不是全部从失配的靶变得不结合。 本发明还涉及将所述寡核苷酸用于研究试剂和临床诊断的方法。 示例性寡核苷酸包含第一可杂交结构域，第二桥连嵌段结构域和第三结合结构域。

公开(公告)号：US20140193815A1

公开(公告)日：2014-07-10

申请号：US14153261

申请日：2014-01-13

Applicant: Gen-Probe Incorporated

Inventor： Lyle J. Arnold, JR. ,  Bob D. Brown

IPC: C12Q1/68

CPC classification number: C12Q1/6834 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2525/197 ,  C12Q2525/161

Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.

Abstract translation: 本发明涉及包含分子开关的寡核苷酸，分子开关可以存在于“开放”或“闭合”位置。 探针的分子开关部分对与分子开关互补的序列的身份特别敏感。 含有分子开关的寡核苷酸适用于各种杂交过程。 由于寡核苷酸的开关结构域的灵敏度，含有分子开关的探针在识别单点错配中特别有用。 更具体地，寡核苷酸的一部分但不是全部从失配的靶变得不结合。 本发明还涉及将所述寡核苷酸用于研究试剂和临床诊断的方法。 示例性寡核苷酸包含第一可杂交结构域，第二桥连嵌段结构域和第三结合结构域。