公开(公告)号：US09243286B2

公开(公告)日：2016-01-26

申请号：US14153261

申请日：2014-01-13

Applicant: Gen-Probe Incorporated

Inventor： Lyle J. Arnold, Jr. ,  Bob D. Brown

IPC: C12Q1/68

CPC classification number: C12Q1/6834 ,  C12Q1/6816 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2525/197 ,  C12Q2525/161

Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.

Abstract translation: 本发明涉及包含分子开关的寡核苷酸，分子开关可以存在于“开放”或“闭合”位置。 探针的分子开关部分对与分子开关互补的序列的身份特别敏感。 含有分子开关的寡核苷酸适用于各种杂交过程。 由于寡核苷酸的开关结构域的灵敏度，含有分子开关的探针在识别单点错配中特别有用。 更具体地，寡核苷酸的一部分但不是全部从失配的靶变得不结合。 本发明还涉及将所述寡核苷酸用于研究试剂和临床诊断的方法。 示例性寡核苷酸包含第一可杂交结构域，第二桥连嵌段结构域和第三结合结构域。

公开(公告)号：US11859238B2

公开(公告)日：2024-01-02

申请号：US16241778

申请日：2019-01-07

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Lizhong Dai ,  Steven Phelps ,  Jijumon Chelliserry

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/70 ,  C12P19/34 ,  C12Q1/6865 ,  C12Q1/6825

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q1/6865 ,  C12Q2537/101 ,  C12Q2545/10

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

公开(公告)号：US09677135B2

公开(公告)日：2017-06-13

申请号：US14109709

申请日：2013-12-17

Applicant: Gen-Probe Incorporated

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Michael M. Becker

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

公开(公告)号：US10724085B2

公开(公告)日：2020-07-28

申请号：US15902819

申请日：2018-02-22

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6853

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US10119163B2

公开(公告)日：2018-11-06

申请号：US15058702

申请日：2016-03-02

Applicant: GEN-PROBE INCORPORATED

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/6865 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6853

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US09139870B2

公开(公告)日：2015-09-22

申请号：US14014607

申请日：2013-08-30

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Lizhong Dai ,  Steven Phelps ,  Jijumon Chelliserry

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/70

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q2537/101 ,  C12Q2545/10

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

Abstract translation: 公开了用于核酸扩增的改进方法，包括多重扩增，其中扩增在两个或多个不同阶段中进行。 第一相扩增反应优选缺少指数扩增所需的一种或多种组分。 随后在第二，第三或另外的扩增相中提供缺乏的组分，导致快速指数扩增反应。 多相协议在低分析物浓度下导致更快更灵敏的检测和更低的变异性。 还公开了用于实施所要求保护的方法的组合物。

公开(公告)号：US10196674B2

公开(公告)日：2019-02-05

申请号：US14856339

申请日：2015-09-16

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Lizhong Dai ,  Steven Phelps ,  Jijumon Chelliserry

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/70 ,  C12P19/34 ,  C12Q1/6865 ,  C12Q1/6825

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

公开(公告)号：US09399796B2

公开(公告)日：2016-07-26

申请号：US13956158

申请日：2013-07-31

Applicant: Gen-Probe Incorporated

Inventor： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.