公开(公告)号：US20130078664A1

公开(公告)日：2013-03-28

申请号：US13673932

申请日：2012-11-09

Applicant: General Atomics

Inventor： Chong-Sheng YUAN ,  Abhijit DATTA ,  Limin LIU

IPC: C12Q1/28

CPC classification number: C12Q1/28 ,  G01N33/723

Abstract: The invention provides enzymatic methods for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The methods utilizes one or two different types of oxidizing agents which selectively oxidize low-molecular weight reducing substances and high-molecular weight (mainly hemoglobin) reducing substances in blood samples, coupled with enzymatic reactions catalyzed by proteases, fructosyl amino acid oxidase. The amount of hydrogen peroxide generated in the reaction is measured for determination of percentage of glycated hemoglobin in blood samples. The invention provides kits for performing the methods of the invention.

Abstract translation: 本发明提供用于直接测定血液样品中糖化血红蛋白百分比的酶法，而不需要分离测量血液样品中的总血红蛋白含量。 该方法使用一种或两种不同类型的氧化剂，其选择性地氧化血液样品中的低分子量还原物质和高分子量（主要是血红蛋白）还原物质，加上由蛋白酶，果糖基氨基酸氧化酶催化的酶反应。 测定反应中产生的过氧化氢的量，以测定血液样品中糖化血红蛋白的百分比。 本发明提供了用于实施本发明方法的试剂盒。