公开(公告)号：US20210371836A1

公开(公告)日：2021-12-02

申请号：US17204449

申请日：2021-03-17

Applicant: Genomatica, Inc.

Inventor： Louis HOM ,  Na TRINH ,  Murtaza ALIBHAI ,  Zhihao HU ,  Eli GROBAN ,  Vikranth ARLAGADDA ,  Elizabeth CLARKE

IPC: C12N9/16 ,  C12N9/18 ,  C12N15/00 ,  C12N15/63

Abstract: The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

公开(公告)号：US20220090028A1

公开(公告)日：2022-03-24

申请号：US17405685

申请日：2021-08-18

Applicant: Genomatica, Inc.

Inventor： Mathew RUDE ,  Na TRINH ,  Andreas SCHIRMER ,  Jacob GANO

IPC: C12N9/02 ,  C12P7/24 ,  C12P7/04 ,  C12P7/64

Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.