公开(公告)号：US20210371836A1

公开(公告)日：2021-12-02

申请号：US17204449

申请日：2021-03-17

Applicant: Genomatica, Inc.

Inventor： Louis HOM ,  Na TRINH ,  Murtaza ALIBHAI ,  Zhihao HU ,  Eli GROBAN ,  Vikranth ARLAGADDA ,  Elizabeth CLARKE

IPC: C12N9/16 ,  C12N9/18 ,  C12N15/00 ,  C12N15/63

Abstract: The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

公开(公告)号：US20210348173A1

公开(公告)日：2021-11-11

申请号：US17335812

申请日：2021-06-01

Applicant: Genomatica, Inc.

Inventor： Derek L. GREENFIELD ,  Andreas W. SCHIRMER ,  Elizabeth J. CLARKE ,  Eli S. GROBAN ,  Bernardo M. DA COSTA ,  Zhihao HU ,  Kevin HOLDEN ,  Noah HELMAN

IPC: C12N15/70 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N9/00 ,  C12P7/04 ,  C12P7/64

Abstract: The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.

公开(公告)号：US20210324430A1

公开(公告)日：2021-10-21

申请号：US17164025

申请日：2021-02-01

Applicant: Genomatica, Inc.

Inventor： Zhihao HU ,  Vikranth ARLAGADDA

IPC: C12P7/64 ,  C12N1/20 ,  B21D51/26 ,  C12P7/04

Abstract: Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.

公开(公告)号：US20210171987A1

公开(公告)日：2021-06-10

申请号：US17074017

申请日：2020-10-19

Applicant: Genomatica, Inc.

Inventor： Jay D. KEASLING ,  Zhihao HU ,  Chris SOMERVILLE ,  George CHURCH ,  David BERRY ,  Lisa C. FRIEDMAN ,  Andreas SCHIRMER ,  Shane BRUBAKER ,  Stephen B. DEL CARDAYRE

IPC: C12P7/04 ,  C10L1/02 ,  C10L1/32 ,  C12P7/64 ,  C07C31/125 ,  C07C33/02

Abstract: Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.

公开(公告)号：US20250101471A1

公开(公告)日：2025-03-27

申请号：US18800482

申请日：2024-08-12

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Noah HELMAN ,  Haibo WANG ,  Zhihao HU ,  Vikranth ARLAGADDA ,  Alma Itzel RAMOS-SOLIS ,  Elizabeth J. CLARKE

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/16 ,  C12N15/52

Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

公开(公告)号：US20250092355A1

公开(公告)日：2025-03-20

申请号：US18816019

申请日：2024-08-27

Applicant: Genomatica, Inc.

Inventor： Derek L. GREENFIELD ,  Louis G. HOM ,  Fernando A. SANCHEZ-RIERA ,  Zhihao HU ,  Vikranth ARLAGADDA ,  Eli S. GROBAN ,  Scott A. FRYKMAN

IPC: C12N1/20 ,  C07C31/125 ,  C07C33/02 ,  C07C53/126 ,  C07K14/195 ,  C07K14/24 ,  C07K14/245 ,  C07K14/255 ,  C07K14/26 ,  C12P7/6409

Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.

公开(公告)号：US20220267813A1

公开(公告)日：2022-08-25

申请号：US17732799

申请日：2022-04-29

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Noah HELMAN ,  Haibo WANG ,  Zhihao HU ,  Vikranth ARLAGADDA ,  Alma Itzel RAMOS-SOLIS ,  Elizabeth CLARKE

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/16 ,  C12N15/52

Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

公开(公告)号：US20210371889A1

公开(公告)日：2021-12-02

申请号：US17350690

申请日：2021-06-17

Applicant: Genomatica, Inc.

Inventor： Derek L. GREENFIELD ,  Louis G. HOM ,  Fernando A. SANCHEZ-RIERA ,  Zhihao HU ,  Vikranth ARLAGADDA ,  Eli S. GROBAN ,  Scott A. FRYKMAN

IPC: C12P7/64 ,  C07K14/245 ,  C07K14/195 ,  C07C31/125 ,  C07C33/02 ,  C07C53/126

Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.

公开(公告)号：US20250019731A1

公开(公告)日：2025-01-16

申请号：US18595947

申请日：2024-03-05

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Haibo WANG ,  Stephen B. DEL CARDAYRE ,  Zhihao HU ,  Louis G. HOM ,  Baolong ZHU ,  Cindy CHANG ,  Emanuela E. POPOVA

IPC: C12P7/6409 ,  C12N9/02 ,  C12N9/04 ,  C12N15/52 ,  C12N15/62 ,  C12P7/18 ,  C12P7/42 ,  C12P7/6436

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US20210355071A1

公开(公告)日：2021-11-18

申请号：US17308864

申请日：2021-05-05

Applicant: Genomatica, Inc.

Inventor： Zhihao HU ,  Fernando VALLE

IPC: C07C69/533 ,  C12N1/20 ,  C12N1/16 ,  C12N1/14 ,  C12N1/12 ,  C07C69/34 ,  C07C69/52 ,  C10L1/02 ,  C10L1/19 ,  C11C3/00 ,  C12P7/64 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/00

Abstract: Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.