公开(公告)号：US20230001412A1

公开(公告)日：2023-01-05

申请号：US17782598

申请日：2020-01-21

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander GOVYADINOV ,  Hilary Ely ,  Brett E DAHLGREN ,  Si-lam J. CHOY ,  Erik TORNIAINEN

IPC: B01L3/00 ,  C12Q1/6844 ,  B01L7/00 ,  G01N21/64

Abstract: A microfluidic reaction chamber with a reaction chamber circuit includes a microfluidic reaction chamber to contain a reaction fluid for amplification of nucleic acids, and a reaction chamber circuit disposed within the microfluidic reaction chamber. The microfluidic reaction chamber includes a base wall, a top wall parallel to the base wall and defined in part by a transparent lid, a first side wall, and a second side wall. The reaction chamber circuit is disposed within the microfluidic reaction chamber, and includes a top surface, a bottom surface, a first side wall, and a second side wall. The reaction chamber circuit is in fluidic contact with the reaction fluid and includes a photodetector to detect a fluorescence signal from a labeled fluorescent tag in the reaction fluid.