公开(公告)号：US20230131184A1

公开(公告)日：2023-04-27

申请号：US17912189

申请日：2020-03-30

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Carson DENISON ,  Erik TORNIAINEN ,  Richard SEAVER

IPC: B01L7/00 ,  C12Q1/686

Abstract: Intermittent warming of a biologic sample including a nucleic acid includes receiving at a first end of a channel of a microfluidic device, a biologic sample including a nucleic acid, and warming a subset of a plurality of heating elements disposed adjacent to the channel. The method includes warming the heating elements to a particular temperature of a particular warming and cooling protocol. The method includes moving the biologic sample from the first end of the channel to a second end of the channel opposite the first end at a particular flow rate associated with the warming and cooling protocol, and intermittently warming the biologic sample using the subset of heating elements while the biologic sample moves from the first end of the channel to the second end of the channel.

公开(公告)号：US20230001412A1

公开(公告)日：2023-01-05

申请号：US17782598

申请日：2020-01-21

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander GOVYADINOV ,  Hilary Ely ,  Brett E DAHLGREN ,  Si-lam J. CHOY ,  Erik TORNIAINEN

IPC: B01L3/00 ,  C12Q1/6844 ,  B01L7/00 ,  G01N21/64

Abstract: A microfluidic reaction chamber with a reaction chamber circuit includes a microfluidic reaction chamber to contain a reaction fluid for amplification of nucleic acids, and a reaction chamber circuit disposed within the microfluidic reaction chamber. The microfluidic reaction chamber includes a base wall, a top wall parallel to the base wall and defined in part by a transparent lid, a first side wall, and a second side wall. The reaction chamber circuit is disposed within the microfluidic reaction chamber, and includes a top surface, a bottom surface, a first side wall, and a second side wall. The reaction chamber circuit is in fluidic contact with the reaction fluid and includes a photodetector to detect a fluorescence signal from a labeled fluorescent tag in the reaction fluid.