公开(公告)号：US08993344B2

公开(公告)日：2015-03-31

申请号：US13258374

申请日：2010-07-16

申请人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

发明人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

IPC分类号： G01N33/53 ,  G01N33/557 ,  G01N33/558

CPC分类号： G01N33/557 ,  G01N33/5306 ,  G01N33/558

摘要： Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently.

摘要翻译： 提供了一种前区现象检测方法，即使使用常规的样本分析工具也能够检测到前区现象的产生，并且可以有效地执行使用免疫色谱法等的检查。

公开(公告)号：US20120015450A1

公开(公告)日：2012-01-19

申请号：US13258374

申请日：2010-07-16

申请人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

发明人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

IPC分类号： G01N33/53 ,  B01J19/00

CPC分类号： G01N33/557 ,  G01N33/5306 ,  G01N33/558

摘要： Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently. In the method, a specimen analysis tool containing substances that specifically bind to a target component contained in a sample is used. The specimen analysis tool is obtained by arranging a sample supplying portion, a reagent portion, and a detection portion on the porous base material from upstream to downstream in a sample moving direction in this order. The reagent portion contains a labeled substance that specifically binds to the target component. The detection portion contains an immobilized substance that specifically binds to the target component. The target component is detected by detecting a complex of the target component, the labeled substance, and the immobilized substance through detection of a label of the labeled substance in the detection portion. The method includes at least one of the following processes A and B: the process A: a process in which detection results obtained in the detection portion are plotted along the sample moving direction, and generation of a prozone phenomenon is detected on the basis of a position of a peak in plots thus obtained; and the process B: a process in which the label is detected at two or more different time points in the detection portion, and generation of a prozone phenomenon is detected on the basis of a magnitude relationship between two or more detection results thus obtained.

摘要翻译： 提供了一种前区现象检测方法，即使使用常规的样本分析工具也能够检测到前区现象的产生，并且可以有效地执行使用免疫色谱法等的检查。 在该方法中，使用含有与试样中含有的靶成分特异结合的物质的试样分析工具。 样品分析工具通过在样品移动方向的上游到下游依次布置多孔基材上的样品供应部分，试剂部分和检测部分而获得。 试剂部分含有与目标成分特异性结合的标记物质。 检测部分含有特异性结合目标成分的固定化物质。 通过检测检测部中的标记物质的标签，检测目标成分，标记物质和固定化物质的复合物来检测目标成分。 该方法包括以下过程A和B中的至少一个：处理A：沿着样品移动方向绘制在检测部分中获得的检测结果的过程，并且基于 如此获得的图中峰的位置; 过程B：在检测部分中的两个或更多个不同时间点检测标签的过程，并且基于由此获得的两个或更多个检测结果之间的大小关系来检测前区现象的产生。

公开(公告)号：US20080194020A1

公开(公告)日：2008-08-14

申请号：US11574641

申请日：2005-09-02

申请人： Jun Imai ,  Mikako Maruya ,  Shigeo Koyasu ,  Hironori Hasegawa ,  Ichiro Yahara

发明人： Jun Imai ,  Mikako Maruya ,  Shigeo Koyasu ,  Hironori Hasegawa ,  Ichiro Yahara

IPC分类号： C12N5/06 ,  C07K14/435 ,  C07H21/04 ,  C12N15/63

CPC分类号： C12N5/0639 ,  C12N2501/07

摘要： The present inventors worked on elucidating the mechanism of antigen cross-presentation by dendritic cells, and revealed that foreign antigens taken up in dendritic cells are degraded by proteasomes after undergoing polyubiquitination. Based on the novel finding that polyubiquitination is involved in cross-presentation, the promotion of polyubiquitination was attempted by a number of methods, and the promotion of antigen presentation was confirmed. These methods enable the production of dendritic cells effective for inducing CTL activation.

摘要翻译： 本发明人致力于阐明树突状细胞抗原交叉呈递的机理，并揭示在经历多聚泛素化后，被树突状细胞吸收的外源抗原被蛋白酶体降解。 基于多泛素化涉及交叉表达的新发现，通过多种方法尝试促进多泛素化，证实了抗原呈递的促进作用。 这些方法能够产生有效诱导CTL活化的树突状细胞。

公开(公告)号：US20080064048A1

公开(公告)日：2008-03-13

申请号：US11574612

申请日：2005-09-02

申请人： Jun Imai ,  Mikako Maruya ,  Shigeo Koyasu ,  Hironori Hasegawa ,  Ichiro Yahara

发明人： Jun Imai ,  Mikako Maruya ,  Shigeo Koyasu ,  Hironori Hasegawa ,  Ichiro Yahara

IPC分类号： G01N33/567 ,  C12N5/02

CPC分类号： C12N5/0639 ,  G01N33/5047

摘要： The present inventors worked on elucidating the mechanism of antigen cross-presentation by dendritic cells, and revealed that foreign antigens taken up in dendritic cells are degraded by proteasomes after undergoing polyubiquitination. They discovered a positive correlation between the uptake amount of an antigenic protein by dendritic cells or the level of polyubiquitination and the level of antigen presentation of the antigenic protein. Based on these findings, methods were developed for predicting the intensity of cross-presentation of an anti genic protein by measuring the amount of antigenic protein taken up or polyubiquitinated protein. The use of these methods allows selecting antigenic proteins that are readily presented as antigens from among a number of proteins. The methods of the present invention enable suitable cancer immune vaccines to be provided through selection of more suitable cancer-specific proteins.

摘要翻译： 本发明人致力于阐明树突状细胞抗原交叉呈递的机理，并揭示在经历多聚泛素化后，被树突状细胞吸收的外源抗原被蛋白酶体降解。 他们发现树突状细胞的抗原蛋白摄取量或多聚泛素化水平与抗原蛋白的抗原呈递水平呈正相关。 基于这些发现，开发了通过测量抗原蛋白摄取或多聚泛素化蛋白的量来预测抗基因蛋白的交叉表达强度的方法。 使用这些方法允许从许多蛋白质中选择容易呈现为抗原的抗原蛋白质。 本发明的方法通过选择更合适的癌症特异性蛋白质来提供合适的癌症免疫疫苗。