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公开(公告)号：US5258301A
公开(公告)日：1993-11-02
申请号：US602221
申请日：1990-10-26
CPC分类号： C12N9/12 , C12P19/38 , Y10S435/832
摘要： The present invention concerns a process for producing nucleosides by carrying out the reaction of a base donor, a saccharide residue donor and a phosphoric acid donor by the use of an enzyme preparation containing nucleoside phosphorylase, thereby forming an N-glycosidic bond between the base moiety of the base donor and the saccharide moiety of the saccharide residue donor, which comprises using, as the enzyme preparation containing nucleoside phosphorylase, a preparation derived from the cells of one or more kinds of microorganisms belonging to thermophiles of the genus Bacillus and having high nucleoside phosphorylase activity per unit cell weight.
摘要翻译： PCT No.PCT / JP90 / 00240 Sec。 371 1990年10月26日第 102（e）1990年10月26日PCT PCT 1990年2月27日PCT公布。 No.W090 / 10080 PCT出版物 日本1990年7月9日。本发明涉及通过使用含有核苷磷酸化酶的酶制剂进行碱供体，糖残基供体和磷酸供体的反应来制备核苷的方法，从而形成N 碱供体的碱基部分和糖残基供体的糖部分之间的糖苷键，其包括使用含有核苷磷酸化酶的酶制剂作为源自嗜热菌的一种或多种微生物的细胞的制剂 芽孢杆菌属，每单位细胞重量具有高核苷磷酸化酶活性。
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公开(公告)号：US5384251A
公开(公告)日：1995-01-24
申请号：US56745
申请日：1993-05-04
CPC分类号： C12N9/12 , C12P19/38 , Y10S435/832
摘要： The present invention concerns a process for producing nucleosides by carrying out the reaction of a base donor, a saccharide residue donor and a phosphoric acid donor by the use of an enzyme preparation containing nucleoside phosphorylase, thereby forming an N-glycosidic bond between the base moiety of the base donor and the saccharide moiety of the saccharide residue donor, which comprises using, as the enzyme preparation containing nucleoside phosphorylase, a preparation derived from the cells of one or more kinds of microorganisms belonging to thermophiles of the genus Bacillus and having high nucleoside phosphorylase activity per unit cell weight.
摘要翻译： 本发明涉及通过使用含有核苷磷酸化酶的酶制剂进行碱供体，糖残基供体和磷酸供体的反应来制备核苷的方法，由此在碱部分之间形成N-糖苷键 的碱供体和糖残基供体的糖部分，其包括使用含有核苷磷酸化酶的酶制剂作为源自属于芽孢杆菌属嗜热菌的一种或多种微生物的细胞的制剂并具有高核苷的制剂 每单位细胞重量的磷酸化酶活性。
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公开(公告)号：US5506122A
公开(公告)日：1996-04-09
申请号：US319574
申请日：1994-10-07
CPC分类号： C12N9/12 , C12P19/38 , Y10S435/832
摘要： The present invention concerns a process for producing nucleosides by carrying out the reaction of a base donor, a saccharide residue donor and a phosphoric acid donor by the use of an enzyme preparation containing nucleoside phosphorylase, thereby forming an N-glycosidic bond between the base moiety of the base donor and the saccharide moiety of the saccharide residue donor, which comprises using, as the enzyme preparation containing nucleoside phosphorylase, a preparation derived from the cells of one or more kinds of microorganisms belonging to thermophiles of the genus Bacillus and having high nucleoside phosphorylase activity per unit cell weight.
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