公开(公告)号：US11414658B2

公开(公告)日：2022-08-16

申请号：US16710632

申请日：2019-12-11

Applicant: Industrial Technology Research Institute

Inventor： Kai-Chun Fan ,  Yin-Lung Han ,  Pei-Jyuan Gao ,  Yong-Yang Lin ,  Chieh-Lun Cheng ,  Chien-Chang Huang ,  Yung-Ho Chang ,  Chia-Long Lin ,  I-Son Ng ,  Bo-Han Chen

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/6876

Abstract: A tracer particle is provided. The tracer particle includes: a core structure; a nucleic acid molecule immobilized on the core structure; and a shell layer covering the core structure and the nucleic acid molecule; wherein the core structure has a first porosity, the shell layer has a second porosity, and the first porosity is greater than the second porosity.

公开(公告)号：US09790463B2

公开(公告)日：2017-10-17

申请号：US14229596

申请日：2014-03-28

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Yin-Lung Han ,  Tai-Rong Guo ,  Jo-Shu Chang ,  Yung-Chong Lou ,  Chieh-Lun Cheng ,  Wan-Ju Yu ,  Chih-Hsi Liu

IPC: C12N1/20

CPC classification number: C12N1/20

Abstract: The disclosure provides a culturing medium for culturing a bacterium of genus Tepidimonas, including: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH4)2SO4), ammonium nitrate (NH4NO3), ammonium chloride (NH4Cl) and urea; phosphate; magnesium chloride (MgCl2); yeast extract; and trace elements.

公开(公告)号：US12194137B2

公开(公告)日：2025-01-14

申请号：US17513484

申请日：2021-10-28

Applicant: Industrial Technology Research Institute

Inventor： Yin-Lung Han ,  Chieh-Lun Cheng ,  Kai-Chun Fan ,  Pei-Jyuan Gao ,  Bo-Han Chen

IPC: A61K8/99 ,  A61K8/65 ,  A61K8/66 ,  A61K8/9778 ,  A61K8/9789 ,  A61K8/98 ,  A61Q5/04

Abstract: A biological hair shape change composition including a macromolecular component, a small molecular component and an alkali agent component is provided. The macromolecular component includes a protease belonging to the class of alkaline proteases, and the small molecular component includes a peptide with reduction activity. Both the macromolecular component and the small molecular component are obtained from a fermentation product of Bacillus licheniformis and a keratin and/or keratin polymer-containing medium. Molecular weights of ingredients in the macromolecular component are greater than or equal to 3 kDa and are 3-1000 kDa, and molecular weights of ingredients in the small molecular component are less than 3 kDa and are 0.01-2.99 kDa.

公开(公告)号：US09580738B2

公开(公告)日：2017-02-28

申请号：US14838612

申请日：2015-08-28

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Yin-Lung Han ,  Jo-Shu Chang ,  Yung-Chong Lou ,  Chieh-Lun Cheng ,  Chih-Hsi Liu

IPC: C12P21/00

CPC classification number: C12P21/00

Abstract: The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.

Abstract translation: 本发明提供了从Tepidimonas属产生细胞外蛋白的方法，包括：用培养基对培养培养基进行发酵培养以形成发酵液，其中培养基的组成包括作为有机物的碳源 酸，选自乙酸酯，乳酸和丁酸酯; 选自（NH 4）2 SO 4，NH 4 NO 3，NH 4 Cl和尿素的氮源; 磷酸盐 碳酸盐 MgCl2; 酵母抽提物; 和微量元素，其中发酵培养的气体进料速率为约0-0.1vvm; 在发酵培养完成后，收集发酵液，其中发酵液含有从Tepidimonas属细菌分泌的细胞外蛋白质。