公开(公告)号：US09790463B2

公开(公告)日：2017-10-17

申请号：US14229596

申请日：2014-03-28

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Yin-Lung Han ,  Tai-Rong Guo ,  Jo-Shu Chang ,  Yung-Chong Lou ,  Chieh-Lun Cheng ,  Wan-Ju Yu ,  Chih-Hsi Liu

IPC: C12N1/20

CPC classification number: C12N1/20

Abstract: The disclosure provides a culturing medium for culturing a bacterium of genus Tepidimonas, including: a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of ammonium sulfate ((NH4)2SO4), ammonium nitrate (NH4NO3), ammonium chloride (NH4Cl) and urea; phosphate; magnesium chloride (MgCl2); yeast extract; and trace elements.

公开(公告)号：US09580738B2

公开(公告)日：2017-02-28

申请号：US14838612

申请日：2015-08-28

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Yin-Lung Han ,  Jo-Shu Chang ,  Yung-Chong Lou ,  Chieh-Lun Cheng ,  Chih-Hsi Liu

IPC: C12P21/00

CPC classification number: C12P21/00

Abstract: The disclosure provides a method for producing extracellular proteins from genus Tepidimonas, including: performing a fermentation culturing to a bacteria of genus Tepidimonas with a culturing medium to form a fermented liquid, wherein the composition of the culturing medium includes a carbon source which is an organic acid, selected from a group consisting of acetate, lactate and butyrate; a nitrogen source selected from a group consisting of (NH4)2SO4, NH4NO3, NH4Cl and urea; phosphate; carbonate; MgCl2; yeast extract; and trace elements, and wherein the gas feeding rate for the fermentation culturing is about 0-0.1 vvm; and after the fermentation culturing is completed, collecting the fermented liquid, wherein the fermented liquid contains extracellular protein secreted from the bacteria of genus Tepidimonas.

Abstract translation: 本发明提供了从Tepidimonas属产生细胞外蛋白的方法，包括：用培养基对培养培养基进行发酵培养以形成发酵液，其中培养基的组成包括作为有机物的碳源 酸，选自乙酸酯，乳酸和丁酸酯; 选自（NH 4）2 SO 4，NH 4 NO 3，NH 4 Cl和尿素的氮源; 磷酸盐 碳酸盐 MgCl2; 酵母抽提物; 和微量元素，其中发酵培养的气体进料速率为约0-0.1vvm; 在发酵培养完成后，收集发酵液，其中发酵液含有从Tepidimonas属细菌分泌的细胞外蛋白质。