公开(公告)号：US20220228110A1

公开(公告)日：2022-07-21

申请号：US17564729

申请日：2021-12-29

Applicant: Industrial Technology Research Institute

Inventor： Yen-Ju LIN ,  Mei-Wei LIN ,  Min-Yuan CHOU

IPC: C12N5/00 ,  C12N15/85 ,  G01N33/50 ,  C07K14/47

Abstract: A method for screening host cells expressing a target protein is provided. The method includes the following steps: providing an expression vector, the expression vector including a promoter, a gene encoding a target protein and an FTH1 gene; transfecting the host cells with the expression vector; culturing the host cells in a medium; and adding iron ions to the medium, and screening the surviving host cells to obtain the host cells expressing the target protein. An expression vector and a method for establishing a cell line stably expressing an exogenous recombinant gene are also provided.

公开(公告)号：US20170159051A1

公开(公告)日：2017-06-08

申请号：US15322878

申请日：2015-08-05

Applicant: Industrial Technology Research Institute ,  Academia Sinica

Inventor： Yen-Ju LIN ,  Jui-Wen HUANG ,  Fu-Tong LIU ,  Huan-Yuan CHEN ,  Wei-Chen HSIEH

IPC: C12N15/113

CPC classification number: C12N15/113 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3521

Abstract: A small interfering RNA is provided. The small interfering RNA consists of a passenger strand and a guide strand, wherein the sequence of the passenger strand comprises the sequence of SEQ ID NO. 7, and the sequence of the guide strand comprises the sequence of SEQ ID NO. 8, wherein the small interfering RNA is capable of inhibiting galectin-12 expression.