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公开(公告)号：US4788144A
公开(公告)日：1988-11-29
申请号：US751976
申请日：1985-06-28
申请人： James R. McMullen
发明人： James R. McMullen
CPC分类号： C07K14/61 , Y10S435/813 , Y10S435/818
摘要： A high-density fermentation process for high yield production of swine growth hormone by transformant E. coli is described. The process employs transformant strains of E. coli containing an expression vector coding for swine growth hormone under the control of a bacteriophage lambda promoter-operator and an expression vector coding for the cI857 temperature-sensitive repressor protein. In the initial growth period, the level of dissolved oxygen in the fermentation medium is maintained at about 20% to 60% saturation and the temperature of the medium is kept between 26.degree. C. and 30.degree. C. Production of swine growth hormone is then induced by raising the temperature of the medium to about 42.degree. C. The temperature is then reduced to about 40.degree. C. to optimize cell growth for the remainder of the induction period, during which the level of dissolved oxygen in the medium is maintained at about 10% to 40% saturation.
摘要翻译： 描述了通过转化体大肠杆菌高产量生产猪生长激素的高密度发酵方法。 该方法使用含有编码猪生长激素的表达载体的大肠杆菌转化体菌株，其在噬菌体λ启动子操纵子和编码cI857温度敏感性阻遏物蛋白质的表达载体的控制下。 在初始生长期，发酵培养基中的溶解氧水平维持在约20％至60％的饱和度，培养基的温度保持在26℃至30℃之间。然后产生猪生长激素 通过将培养基的温度升高至约42℃来诱导。然后将温度降低至约40℃，以在诱导期的剩余时间内优化细胞生长，在此期间培养基中的溶解氧水平维持在 约10％至40％的饱和度。
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公开(公告)号：US5849550A
公开(公告)日：1998-12-15
申请号：US497397
申请日：1995-06-30
CPC分类号： C12P17/162
摘要： A method for producing lysocellin in bead form comprises: (a) cultivating a lysocellin-producing microorganism in an aqueous nutrient-containing fermentation broth under lysocellin-producing conditions; (b) adding a base or inorganic phosphate to the fermentation broth within about the first 24 hours of the beginning of the fermentation in an amount sufficient to ensure that the lysocellin is produced in the form of a cation salt of lysocellin; (c) providing a lipid to the fermentation broth such that the lipid is present in the broth in a sufficient amount to form discrete beads with the lysocellin in the fermentation broth; and (d) separating the beads from the broth.
摘要翻译： 用于制备珠粒形式的溶血细胞素的方法包括：（a）在产生溶血细胞素的条件下，在含水营养的发酵液中培养产生溶血细胞纤维素的微生物; （b）在发酵开始的最初24小时之内，将发酵液中的碱或无机磷酸盐以足以确保溶血细胞素的阳离子盐形式产生的量的量加入到发酵液中; （c）向发酵液提供脂质，使得脂肪以足够的量存在于肉汤中，以在发酵液中与溶菌酶一起形成离散的珠粒; 和（d）从培养液中分离珠子。
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公开(公告)号：US20150371356A1
公开(公告)日：2015-12-24
申请号：US14310519
申请日：2014-06-20
申请人： James R. McMullen
发明人： James R. McMullen
IPC分类号： G06T1/20
CPC分类号： H04M1/72533 , H04M1/72527
摘要： A phone display enhancing connection device connects a phone to an extrinsic electronic device having a larger display screen. The device includes a cord having a first end for electronic coupling to a phone and a second end for electronic coupling to an extrinsic electronic apparatus having a display screen larger than a display screen of the phone. A housing is positioned between a first section of the cord and a second section of the cord. A processor positioned in the housing is communicatively coupled between the first end of the cord and the second end of the cord. The processor has translation programming wherein the processor is configured to translate communications between the phone and the extrinsic electronic apparatus for displaying images from the phone on the extrinsic electronic apparatus and controlling the phone using the extrinsic electronic apparatus.
摘要翻译： 电话显示增强连接装置将电话连接到具有较大显示屏的外在电子设备。 该装置包括具有用于电子耦合到电话的第一端的电缆和用于电子耦合到具有大于电话的显示屏的显示屏的外在电子设备的第二端。 壳体位于帘线的第一部分和帘线的第二部分之间。 定位在壳体中的处理器通信地连接在电线的第一端和电线的第二端之间。 处理器具有翻译程序，其中处理器被配置为翻译电话和外在电子设备之间的通信，用于在外部电子设备上显示来自电话的图像，并使用外在电子设备来控制电话。
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